Igf2 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:75952 Davies K, et al., Disruption of mesodermal enhancers for Igf2 in the minute mutant. Development. 2002 Apr;129(7):1657-68
Assay type:	Northern blot
MGI Accession ID:	MGI:2178261
Gene symbol:	Igf2
Gene name:	insulin-like growth factor 2

Probe:	Probe 6
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	3.8 kb	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Embryo Mnt^p	E13.5 (a)	TS21: embryo	Present (b)	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	Minute/Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Embryo ^m	E13.5 (a)	TS21: embryo	Present (c)	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	Minute/Minute⁺	Not Specified	The minute allele was inherited via maternal transmission.
Embryo +	E13.5 (a)	TS21: embryo	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Embryo Hom	E13.5 (a)	TS21: embryo	Present (d)	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	Minute/Minute	Not Specified	The minute alleles were transmitted both paternally and maternally.
Placenta ^p	E13.5 (a)	TS28: placenta	Absent	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	Minute/Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Placenta ^m	E13.5 (a)	TS28: placenta	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	Minute/Minute⁺	Not Specified	The minute allele was inherited via maternal transmission.
Placenta +	E13.5 (a)	TS28: placenta	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Placenta Hom	E13.5 (a)	TS28: placenta	Absent	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	Minute/Minute	Not Specified	The minute alleles were transmitted both paternally and maternally.

Notes:
(a) Age normalized so that noon of plug day = E0.5
(b) Authors state that expression is substantially reduced (18% of wild type).
(c) Authors report normal expression at this stage.
(d) Authors state that expression is substantially reduced (38% of wild type).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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