Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:73775
Byrd N, et al., Hedgehog is required for murine yolk sac angiogenesis. Development. 2002 Jan;129(2):361-72
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:2177620
|
|
Gene symbol:
|
Kdr
|
|
Gene name:
|
kinase insert domain protein receptor
|
|
Antibody:
|
anti-Flk-1 (Clone Avas 12alpha1)
|
|
Detection system:
|
Secondary antibody coupled to horseradish peroxidase
|
|
Assay notes:
|
The Ihh mutation was either generated in CJ7 or R1 ES cells and these were injected into C57BL/6 mice. Chimeric males were mated to C57BL/6, 129/Sv, or Swiss Webster females. Heterozygotes were maintained on a non-specified background.
|
|
|
Results
|
Specimen
6B, +/-:
embryonic day 11.5; Ihhtm1Amc/Ihh +
(more  )
Specimen
6B, +/-:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Ihhtm1Amc/Ihh+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
6B, -/-:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Ihhtm1Amc/Ihhtm1Amc
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools