Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73506
Lowe LA, et al., Genetic dissection of nodal function in patterning the mouse embryo. Development. 2001 May;128(10):1831-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2159422
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe31
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Heterozygote Nodal mutants at E8.5 are defined as Type I, II or III mutants depending on the penetrance of the phenotype.
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Results
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Specimen
5. A.:
embryonic day 8.5
(more  )
Specimen
5. A.:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE. Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5. B.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a type I mutant.
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Specimen
5. C.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a type II mutant.
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Specimen
5. D.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a type II mutant.
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Specimen
5. E.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a type III mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
Regionally restricted |
5E
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Expression is detected along the anterior-posterior axis.
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Specimen
5. F.:
embryonic day 8.5
(more )
Note: Specimen 5. F. is a section of the 5. A. whole-mount.
Specimen
5. F.:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE. Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen 5. F. is a section of the 5. A. whole-mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochord |
Present |
Regionally restricted |
5F
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Expression is detected in the notochord of the hindbrain and the prechordal plate of the forebrain.
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| TS12: notochordal plate |
Present |
Regionally restricted |
5F
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Expression is detected in the notochord of the hindbrain and the prechordal plate of the forebrain.
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Specimen
5. G.:
embryonic day 8.5; Nodaltm1Mku/ Nodaltm1.1Mku
(more )
Note: Specimen 5. G. is a section of the 5. B. whole-mount.
Specimen
5. G.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen 5. G. is a section of the 5. B. whole-mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
Regionally restricted |
5G
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Expression is detected in the notochord of the hindbrain.
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| TS13: notochordal plate |
Absent |
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5G
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No expression was detected in the prechordal plate of the forebrain due to absence of the structure.
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Specimen
5. H.:
embryonic day 8.5; Nodaltm1Mku/ Nodaltm1.1Mku
(more )
Note: Specimen 5. H. is a section of the 5 C. whole-mount.
Specimen
5. H.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen 5. H. is a section of the 5 C. whole-mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
Regionally restricted |
5H
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Expression is detected in the trunk notochord and ends at the head level.
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Specimen
5. I.:
embryonic day 8.5
(more )
Note: Specimen 5. I. is a section of the 5. A. whole-mount.
Specimen
5. I.:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE. Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen 5. I. is a section of the 5. A. whole-mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochord |
Present |
Regionally restricted |
5I
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Expression is detected in the trunk notochord.
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Specimen
5. J.:
embryonic day 8.5; Nodaltm1Mku/ Nodaltm1.1Mku
(more )
Note: Specimen 5. J. is a section of the 5. C. whole-mount.
Specimen
5. J.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen 5. J. is a section of the 5. C. whole-mount.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
Regionally restricted |
5J
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Expression is detected in the trunk notochord.
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Specimen
5. K.:
embryonic day 8.5; Nodaltm1Mku/ Nodaltm1.1Mku
(more )
Note: Specimen 5. K. is a section of the 5. D. whole-mount
Specimen
5. K.:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen 5. K. is a section of the 5. D. whole-mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Absent |
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5K
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Shh-negative type II mutants lacked notochord completely.
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Specimen
5. L:
embryonic day 8.5; Nodaltm1Mku/ Nodaltm1.1Mku
(more )
Note: Specimen 5. L. is a section of the 5. E. whole-mount
Specimen
5. L:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
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Mutant Allele(s):
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Nodaltm1Mku/Nodaltm1.1Mku
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen 5. L. is a section of the 5. E. whole-mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
Regionally restricted |
5L
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Expression is detected in the notochord adjacent to the neural tube.
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