Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:73506
Lowe LA, et al., Genetic dissection of nodal function in patterning the mouse embryo. Development. 2001 May;128(10):1831-43
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2159421
|
|
Gene symbol:
|
Hesx1
|
|
Gene name:
|
homeobox gene expressed in ES cells
|
|
Probe:
|
Hesx1/Rpx probe7
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
3. O.:
embryonic day 7.5
(more )
Specimen
3. O.:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3. P.:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
|
|
Mutant Allele(s):
|
Nodaltm1Mku/Nodaltm1.1Mku
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: anterior visceral endoderm |
Present |
Regionally restricted |
3P
|
Expression is detected in the anterior visceral endoderm and the overlying neural plate but is more distal than in wild-type.
|
| TS11: future spinal cord neural plate |
Present |
Regionally restricted |
3P
|
Expression is detected in the anterior visceral endoderm and the overlying neural plate but is more distal than in wild-type.
|
|