Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73074
Bai CB, et al., Gli1 can rescue the in vivo function of Gli2. Development. 2001 Dec;128(24):5161-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2158413
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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Anti-Isl1/2
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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The authors stated they used an antibody from the Developmental Studies Hybridoma Bank which recognizes both Isl1 and Isl2. However, they did not specify which antibody they used, so instead of clone 40.2D6 (MGI:1314839), it could have been clone 39.4D5 (MGI:2149733).
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Results
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Specimen
2D:
embryonic day 10.5
(more  )
Specimen
2D:
(close  )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2J:
(close )
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Genetic Background:
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either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss)
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Mutant Allele(s):
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Gli2tm2.1Alj/Gli2tm2.1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
2J
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Expression is present in the motoneurons. In the mutant their area of expression is expanded ventrally relative to wild-type.
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Specimen
2M:
embryonic day 10.5
(more )
Specimen
2M:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2N:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * Black Swiss)
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm2.1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
2N
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Expression is present in the motoneurons. In the mutant their area of expression is expanded ventrally relative to wild-type.
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Specimen
6A:
embryonic day 10.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
6A
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Expression is present in the motoneurons in the ventralateral region.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Absent |
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6B
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In the mutant the motoneurons are lost.
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Specimen
6C:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * Black Swiss)
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Mutant Allele(s):
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Gli2tm2.1Alj/Gli2tm2.1Alj
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Absent |
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6C
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In the mutant the motoneurons are lost.
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Analysis Tools