Probe:	Shh probe30
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS25: midbrain tegmentum	Present
TS25: amygdala	Present
TS25: tectum	Absent
TS25: cerebral cortex	Absent

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1K	Expression is present in the neocortex in a layer-specific pattern. Authors indicate it is probably in layer V.
TS27: cingulate cortex	Present		1K
TS27: dentate gyrus	Present		1K

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1L	Expression is present within the cortex, probably in layer V.

Structure	Level	Pattern	Image	Note
TS27: tectum	Present	Regionally restricted	1M	Expression is present in the superficial layers of the tectum.
TS27: cerebellum Purkinje cell layer	Present		1M

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present
TS27: cerebral cortex	Present
TS27: cingulate cortex	Present
TS27: dentate gyrus	Present
TS27: tectum	Present

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebral cortex	Present
TS28: cingulate cortex	Present
TS28: dentate gyrus	Present
TS28: tectum	Present