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Gene Expression Data
Assay Details
Assay
Reference: J:71998 Tremblay KD, et al., Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development. 2001 Sep;128(18):3609-21
Assay type: RNA in situ
MGI Accession ID: MGI:2155945
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Probe: Sox2 cDNA4
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Authors generated two Madh1 targeted mutations, Madh1<tm1Rob> and Madh1<tm2.1Rob>. Phenotype is stated as identical, but the mutation used in the in-situ experiments is not specified.
Results
Specimen 5. D.: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS11: neural ectoderm Present 5D
TS11: trophectoderm Present 5D
TS11: embryo endoderm Present Regionally restricted 5D Expression is detected in the definitive endoderm.
Specimen 5. E.: embryonic day 8.0; Smad1tm2.1Rob/Smad1tm2.1Rob (more )
Structure Level Pattern Image Note
TS12: chorion Present Regionally restricted 5E Expression emanates from the posterior portion of the embryo toward the chorion.
TS12: embryo Present Regionally restricted 5E Expression emanates from the posterior portion of the embryo toward the chorion.
TS12: neural ectoderm Present 5E
TS12: embryo endoderm Present Regionally restricted 5E Expression is detected in the definitive endoderm.
Specimen 5. E'.: embryonic day 8.0; Smad1tm2.1Rob/Smad1tm2.1Rob (more )
Structure Level Pattern Image Note
TS12: neural ectoderm Present 5E'
TS12: embryo endoderm Present Regionally restricted 5E' Expression is detected in the definitive endoderm.
TS12: embryo Present Regionally restricted 5E' Expression is detected in a ring of cells projecting from the posterior.
Specimen 5. E''.: embryonic day 8.0; Smad1tm2.1Rob/Smad1tm2.1Rob (more )
Structure Level Pattern Image Note
TS12: neural ectoderm Present Regionally restricted 5E''
TS12: embryo endoderm Present 5E'' Expression is detected in the definitive endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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