Fgf3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:71998 Tremblay KD, et al., Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development. 2001 Sep;128(18):3609-21
Assay type:	RNA in situ
MGI Accession ID:	MGI:2155907
Gene symbol:	Fgf3
Gene name:	fibroblast growth factor 3

Probe:	int-2f
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Authors generated two Madh1 targeted mutations, Madh1<tm1Rob> and Madh1<tm2.1Rob>. Phenotype is stated as identical but the mutation used in the in-situ experiments is not specified. Authors reference J:9254 for probe details and J:9254 lists two probesas used for in-situ probes, int-2g (MGI:10697) and int-2f (MGI:10698). J:9254 states that identical results were obtained with both probes. We are unable to determine which probe was used in this assay.

Results

4F +/+ ©

Specimen 4. F. +/+: embryonic day 7.0 (more

)

Structure	Level	Pattern	Image	Note
TS10: extraembryonic mesoderm	Present		4F +/+
TS10: primitive streak	Present		4F +/+

4F -/- ©

Specimen 4. F. -/-: embryonic day 7.0; Smad1^tm2.1Rob/Smad1^tm2.1Rob (more

)

Structure	Level	Pattern	Image	Note
TS10: extraembryonic mesoderm	Present		4F -/-
TS10: primitive streak	Present		4F -/-
TS10: extraembryonic visceral endoderm	Present	Regionally restricted	4F -/-	Expression is detected in the folds of the visceral endoderm (VE). Author states this confirms the presence of extra-embryonic mesoderm in the VE folds.

4G ©

Specimen 4. G. -/-: embryonic day 7.0; Smad1^tm2.1Rob/Smad1^tm2.1Rob (more

)
Note: Specimen 4. G. is a cross-section hybridization of the same embryo in specimen 4. F.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic visceral endoderm	Present	Regionally restricted	4G	Expression is detected in the folds of the visceral endoderm (VE). Author states this confirms the presence of extra-embryonic mesoderm in the VE folds.
TS10: extraembryonic mesoderm	Present		4G

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24