Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72725
Martinez-Barbera JP, et al., Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development. 2001 Dec;128(23):4789-800
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2155561
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Gene symbol:
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Gbx2
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Gene name:
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gastrulation brain homeobox 2
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Probe:
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Gbx2 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2B:
embryonic day 8.7
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
Regionally restricted |
2B
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Expression is at the midbrain-hindbrain boundary.
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| TS14: future midbrain |
Present |
Regionally restricted |
2B
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Expression is at the midbrain-hindbrain boundary.
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Specimen
2P:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future brain |
Present |
Regionally restricted |
2P
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The expression domain is caudalized in this mutant.
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Specimen
4B:
embryonic day 9.7
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
4B
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Expression is on the metencephalic side of the midbrain-hindbrain boundary.
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Specimen
4G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx1tm1Asim/Otx1+
Otx2tm1Pas/Otx2+
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
4G
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The domain of expression is expanded anteriorly.
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| TS15: future midbrain |
Present |
Regionally restricted |
4G
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The domain of expression is expanded anteriorly.
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Specimen
5B:
embryonic day 7.5
(more )
Note: Embryo is in the headfold stage.
Specimen
5B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
5B
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The expression is posterior.
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Specimen
5B'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
5B''
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The expression is posterior. Expression in this mutant is the same as in wild-type embryos.
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Specimen
5D:
embryonic day 8.0
(more )
Note: Embryo is in the late headfold stage.
Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the late headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
5D
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The expression is posterior.
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Specimen
5D'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the late headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
5D''
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The expression is posterior. In this mutant, the anterior domain of normal expression is silenced.
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Specimen
5F:
embryonic day 8.0
(more )
Note: Embryo is in the presomite stage.
Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the presomite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
5F
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The expression is posterior.
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Specimen
5F'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Embryo is in the presomite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
5F''
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The expression is posterior. In this mutant, the anterior domain of normal expression is silenced.
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Specimen
5H:
embryonic day 8.0
(more )
Note: 1-2 somite stage.
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1-2 somite stage.
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Specimen
5H'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1-2 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
5H''
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Expression is repressed in the anterior hindbrain.
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Specimen
5J:
embryonic day 8.0
(more )
Note: 4-5 somite stage.
Specimen
5J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-5 somite stage.
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Specimen
5J'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4-5 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
5J''
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Expression is repressed in the rostral hindbrain.
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Specimen
7B:
embryonic day 8.7
(more )
Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
7L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Analysis Tools