Probe:	neuregulin type III
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Strong	Regionally restricted	4J	Expression in the motoneurons located in the ventral coumn of the spinal cord.
TS16: dorsal root ganglion	Strong		4J

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	4K	Expression in the motoneurons located in the ventral coumn of the spinal cord.
TS20: dorsal root ganglion	Strong		4K

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Strong	Regionally restricted	4L	Expression in the motoneurons located in the ventral coumn of the spinal cord.
TS26: dorsal root ganglion	Strong		4L

Structure	Level	Pattern	Image	Note
TS26: brain	Present	Uniform	7D
TS26: hippocampus	Strong		7D
TS26: thalamus	Strong		7D	Expression in the nuclei of the thalamus.
TS26: midbrain	Strong		7D
TS26: retina	Strong		7D
TS26: diencephalon pia mater	Strong		7D
TS26: hindbrain pia mater	Strong		7D
TS26: midbrain pia mater	Strong		7D
TS26: telencephalon pia mater	Strong		7D
TS26: olfactory epithelium	Present		7D

Structure	Level	Pattern	Image	Note
TS20: telencephalon ventricular layer	Absent
TS20: cerebral cortex	Present
TS20: diencephalon	Present
TS20: midbrain	Present
TS20: medulla oblongata	Present
TS20: pons	Present
TS20: spinal cord	Strong
TS20: nasal cavity epithelium	Strong			Expression was strong in undifferentiated epithelium.
TS20: retina	Absent			Absent in undifferentiated epithelium.
TS20: inner ear epithelium	Absent			Absent in undifferentiated epithelium.
TS20: sympathetic ganglion	Present
TS20: skeletal muscle	Absent
TS20: cardiac muscle tissue	Absent
TS20: atrioventricular endocardial cushion	Absent
TS20: endocardial cushion	Absent
TS20: right atrium valve	Absent
TS20: endocardial lining	Weak

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong
TS23: cerebral cortex	Strong
TS23: olfactory lobe	Present
TS23: corpus striatum	Present
TS23: diencephalon	Strong
TS23: midbrain	Strong
TS23: medulla oblongata	Strong
TS23: pons	Strong
TS23: spinal cord	Strong
TS23: choroid plexus	Absent
TS23: pituitary gland	Strong
TS23: olfactory epithelium	Strong
TS23: nasal cavity respiratory epithelium	Absent
TS23: retina nuclear layer	Present	Regionally restricted		Expression was detected in the ganglion cell layer but was absent from the other nuclear layers.
TS23: inner ear epithelium	Absent			Absent in the sensory and non-sensory epithelium.
TS23: sympathetic ganglion	Present
TS23: skeletal muscle	Absent
TS23: cardiac muscle tissue	Absent
TS23: atrioventricular endocardial cushion	Absent
TS23: bulbar cushion	Absent
TS23: endocardial cushion	Absent
TS23: heart valve	Absent
TS23: interventricular septum endocardial lining	Weak
TS23: left atrium auricular region endocardial lining	Weak
TS23: left atrium endocardial lining	Weak
TS23: left ventricle endocardial lining	Weak
TS23: right atrium auricular region endocardial lining	Weak
TS23: right atrium endocardial lining	Weak
TS23: right ventricle endocardial lining	Weak

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex ventricular zone	Strong
TS26: cerebral cortex	Strong
TS26: hippocampus	Strong
TS26: olfactory lobe	Present
TS26: corpus striatum	Strong
TS26: telencephalon	Present	Regionally restricted		Expression was detected in the ventricular zone, cortex, hippocampus, olfactory lobes and striatum but was absent in the claustrum.
TS26: diencephalon	Present
TS26: midbrain	Present
TS26: cerebellum	Strong	Regionally restricted		Expression was detected in the medulla but was absent in the cortex.
TS26: medulla oblongata	Strong
TS26: pons	Strong
TS26: spinal cord	Strong
TS26: choroid plexus	Absent
TS26: pituitary gland	Strong
TS26: olfactory epithelium	Strong
TS26: nasal cavity respiratory epithelium	Absent
TS26: retina nuclear layer	Present	Regionally restricted		Expression was detected in the ganglion cell layer but was absent from the other nuclear layers.
TS26: inner ear epithelium	Absent			Absent in sensory and non-sensory epithelium.
TS26: sympathetic ganglion	Present
TS26: skeletal muscle	Absent
TS26: cardiac muscle tissue	Absent
TS26: atrioventricular endocardial cushion	Absent
TS26: endocardial cushion	Absent
TS26: heart valve	Absent
TS26: interventricular septum endocardial lining	Weak
TS26: left atrium auricular region endocardial lining	Weak
TS26: left atrium endocardial lining	Weak
TS26: left ventricle endocardial lining	Weak
TS26: right atrium auricular region endocardial lining	Weak
TS26: right atrium endocardial lining	Weak
TS26: right ventricle endocardial lining	Weak

Structure	Level	Pattern	Image	Note
TS20: endocardial lining	Absent			Expression was not observed in the endocardium of whole mount embryo with this probe.

Structure	Level	Pattern	Image	Note
TS23: endocardial tissue	Absent			Expression was not observed in the endocardium of whole mount embryo with this probe.

Structure	Level	Pattern	Image	Note
TS26: endocardial tissue	Absent			Expression was not observed in the endocardium of whole mount embryo with this probe.