Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67157
McCright B, et al., Defects in development of the kidney, heart and eye vasculature in mice homozygous for a hypomorphic Notch2 mutation. Development. 2001 Feb;128(4):491-502
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2150127
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Gene symbol:
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Vegfa
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Gene name:
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vascular endothelial growth factor A
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Probe:
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Vegf cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5 G:
embryonic day 16.5
(more  )
Specimen
5 G:
(close  )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: maturing glomerular tuft |
Present |
Regionally restricted |
5G
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Expression in the cup-shaped semicircle of glomerular epithelial cells that are differentiating into podocytes.
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Specimen
5 H:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Notch2tm1Grid/Notch2tm1Grid
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: maturing glomerular tuft |
Present |
Regionally restricted |
5H
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Expressing cells were clumped together in the center of the glomerulus and did not form the cup-shaped epithelial layer observed in the controls.
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Specimen
+/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Notch2tm1Grid/Notch2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools
