Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60855
Birgbauer E, et al., Kinase independent function of EphB receptors in retinal axon pathfinding to the optic disc from dorsal but not ventral retina. Development. 2000 Mar;127(6):1231-41
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2150111
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Gene symbol:
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Efnb2
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Gene name:
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ephrin B2
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Probe:
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ephrinB2 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2B:
embryonic day 13.5
(more  )
Note: Similar results were obtained using CD1 mice.
Specimen
2B:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Similar results were obtained using CD1 mice.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retinal epithelium |
Present |
Regionally restricted |
2B
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina. There was no expression in the retinal ganglion cell layer.
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| TS21: pigmented retinal epithelium |
Present |
Graded |
2B
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina.
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Specimen
2C:
embryonic day 16.5
(more )
Note: Similar results were obtained using CD1 mice.
Specimen
2C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Similar results were obtained using CD1 mice.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina nuclear layer |
Present |
Regionally restricted |
2C
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina. There was no expression in the retinal ganglion cell layer.
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| TS24: pigmented retinal epithelium |
Present |
Graded |
2C
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina.
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| TS24: retina nerve fiber layer |
Present |
Graded |
2C
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Ephb2tm2Paw/Ephb2tm2Paw
Ephb3tm1Kln/Ephb3tm1Kln
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Similar results were obtained using CD1 mice.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina nuclear layer |
Present |
Regionally restricted |
5G
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina. There was no expression in the retinal ganglion cell layer.
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| TS24: pigmented retinal epithelium |
Present |
Graded |
5G
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina.
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| TS24: retina nerve fiber layer |
Present |
Graded |
5G
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Present in the outer retina, including the peripheral margin, in a graded fashion, with higher expression in the dorsal retina compared to the ventral retina.
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