Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62985
Helmbacher F, et al., Targeting of the EphA4 tyrosine kinase receptor affects dorsal/ventral pathfinding of limb motor axons. Development. 2000 Aug;127(15):3313-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2149748
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Gene symbol:
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Epha4
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Gene name:
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Eph receptor A4
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Antibody:
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Sek-1I
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
1D:
embryonic day 11.5
(more  )
Note: Vibratome section.
Specimen
1D:
(close  )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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Specimen
1E:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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Specimen
5B:
embryonic day 11.5
(more )
Note: Vibratome section.
Specimen
5B:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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Specimen
5D:
embryonic day 11.5
(more )
Note: Vibratome section.
Specimen
5D:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: peripheral nervous system |
Strong |
Regionally restricted |
5D
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Axons entering the dorsal compartment of the limb bud were strongly labelled, whereas axons projecting ventrally were only weakly positive.
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| TS19: segmental spinal nerve |
Strong |
Regionally restricted |
5D
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Axons entering the dorsal compartment of the limb bud were strongly labelled, whereas axons projecting ventrally were only weakly positive.
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| TS19: future leg mesenchyme |
Strong |
Regionally restricted |
5D
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Expression is in the proximal part of the dorsal limb bud.
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Specimen
5F:
embryonic day 11.5
(more )
Note: Vibratome section.
Specimen
5F:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Vibratome section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: peripheral nervous system |
Strong |
Regionally restricted |
5F
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Axons entering the dorsal compartment of the limb bud were still strongly labelled, whereas axons projecting ventrally were only weakly positive.
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Specimen
5G:
embryonic day 11.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: peripheral nervous system |
Present |
Regionally restricted |
5G
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Axons entering the dorsal compartment of the limb bud were still strongly labelled, whereas axons projecting ventrally were only weakly positive.
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Analysis Tools