Probe:	alpha-cardiac actin probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	2B	Present in the muscle masses of the neck and in greater amounts than Fgf6.

Structure	Level	Pattern	Image	Note
TS22: shoulder mesenchyme	Present	Regionally restricted	2E	Present in the muscle masses of the shoulder. It is more widely distributed than Fgf6.
TS22: forelimb	Present	Regionally restricted	2E	Present in the muscle masses of the forelimb. It is more widely distributed than Fgf6.

Structure	Level	Pattern	Image	Note
TS22: hindlimb	Present	Regionally restricted	2G	Present in the muscle masses of the hindlimb.

Structure	Level	Pattern	Image	Note
TS22: vertebral axis musculature	Present	Regionally restricted	3B	Present in the muscle masses of the neck.

Structure	Level	Pattern	Image	Note
TS22: shoulder mesenchyme	Present	Regionally restricted	3D	Present in the muscle masses.

Structure	Level	Pattern	Image	Note
TS22: tongue muscle	Present		6C	Expression is present in the tongue in linear arrays (indicative of fibers); it is also present in the fibers surrounding the tongue.