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Gene Expression Data
Assay Details
Assay
Reference: J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
Assay type: RNA in situ
MGI Accession ID: MGI:2148286
Gene symbol: Pax5
Gene name: paired box 5
Probe: 5sg8
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3K: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future midbrain Present 3K
TS15: rhombomere 1 Present 3K
Specimen 3L: embryonic day 9.5; Emx2tm1Sia/Emx2tm1Sia, Otx2tm1Sia/Otx2+ (more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted 3L Enlarged regions of expression both rostral and caudal to isthmic constriction; greatly reduced negative region extending up to the sulcus telodiencephalicus.
TS15: future midbrain Present Regionally restricted 3L Enlarged regions of expression both rostral and caudal to isthmic constriction; greatly reduced negative region extending up to the sulcus telodiencephalicus.
Specimen 4I: embryonic day 8.0 (more )
Note: 6-somite stage.
Structure Level Pattern Image Note
TS12: future midbrain Present Regionally restricted 4I Authors report complete regression to the caudal portion of the prospective mesencephalon.
Specimen 4J: embryonic day 8.0; Emx2tm1Sia/Emx2tm1Sia, Otx2tm1Sia/Otx2+ (more )
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Structure Level Pattern Image Note
TS12: future midbrain Present Regionally restricted 4J Expansion of positive domain accompanied by an anterior shift.
Specimen 8I: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: midbrain Present 8I
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory