Gene Expression Data
Assay Details
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Assay
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Reference:
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J:70747
Tekki-Kessaris N, et al., Hedgehog-dependent oligodendrocyte lineage specification in the telencephalon. Development. 2001 Jul;128(13):2545-54
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2148253
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Probe:
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PDGF-R alpha probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 12.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Present |
Regionally restricted |
6A
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Expression in oligodendrocyte progenitors (OLPs) in the ventral forebrain.
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| TS20: medial ganglionic eminence |
Present |
Scattered |
6A
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Expression in numerous OLPs scattered in the medial ganglionic eminence.
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| TS20: diencephalon |
Present |
Regionally restricted |
6A
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No expression in the zona limitans intrathalamica.
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| TS20: hypothalamus |
Present |
Regionally restricted |
6A
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Expression in numerous OLPs scattered in the anterior hypothalamus.
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Specimen
6C:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * Black Swiss)
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1tm1Shk
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Absent |
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6C
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Expression in numerous OLPs scattered in the anterior hypothalamus.
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Specimen
Nkx2.1(-/-) E14.5:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * Black Swiss)
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1tm1Shk
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forebrain |
Absent |
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Complete absence of Pdgfra-expressing OLPs in the ventral forebrain.
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Specimen
Nkx2.1(-/-) E16.5:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * Black Swiss)
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1tm1Shk
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
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Sparse population of expressing cells.
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Specimen
Nkx2.1(-/-) E18.5:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * Black Swiss)
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1tm1Shk
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: telencephalon |
Present |
Regionally restricted |
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Authors report almost normal numbers of OLPs throughout the cortex and basal ganglia.
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Analysis Tools
