Probe:	Dlx-specific probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1B	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: telencephalon	Present	Regionally restricted	1B	Telencephalic domain includes septum (SE), diagonal band (DB), anterior entopeduncular area (AEP), anterior preooptic area (POA).
TS20: diencephalon	Present	Regionally restricted	1B	Thalamo-hypothalamic domain includes ventral thalamus (VT), posterior entodpeduncular area (PEP), hypothalamic cell cord (HCC), suprachiasmatic area (SCH).
TS20: lateral ganglionic eminence	Present		1B
TS20: medial ganglionic eminence	Present		1B

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1D
TS20: hypothalamus	Present	Regionally restricted	1D	Compartmentalization is disturbed; expression becomes widely disperse and extends beyond normal sharp boundaries.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1F	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: telencephalon	Present	Regionally restricted	1F	Telencephalic domain includes septum (SE), diagonal band (DB), anterior entopeduncular area (AEP), anterior preooptic area (POA).
TS20: diencephalon	Present	Regionally restricted	1F	Thalamo-hypothalamic domain includes ventral thalamus (VT), posterior entodpeduncular area (PEP), hypothalamic cell cord (HCC), suprachiasmatic area (SCH).
TS20: hypothalamus	Present	Regionally restricted	1F	Specifically delineates regions of hypothalamic cell cord (HCC).
TS20: lateral ganglionic eminence	Present		1F
TS20: medial ganglionic eminence	Present		1F

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1H
TS20: hypothalamus	Present	Regionally restricted	1H	Compartmentalization is disturbed; expression becomes widely disperse and extends beyond normal sharp boundaries.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1J	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: telencephalon	Present	Regionally restricted	1J	Telencephalic domain includes septum (SE), diagonal band (DB), anterior entopeduncular area (AEP), anterior preooptic area (POA).
TS20: diencephalon	Present	Regionally restricted	1J	Thalamo-hypothalamic domain includes ventral thalamus (VT), posterior entodpeduncular area (PEP), hypothalamic cell cord (HCC), suprachiasmatic area (SCH).
TS20: thalamus	Present	Regionally restricted	1J	Domain includes regions of ventral thalamus (VT), but not the dorsalmost region of the VT.
TS20: hypothalamus	Present	Regionally restricted	1J	Specifically delineates regions of hypothalamic cell cord (HCC).
TS20: lateral ganglionic eminence	Present		1J
TS20: medial ganglionic eminence	Present		1J

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1L

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1N	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: diencephalon	Present	Regionally restricted	1N	Thalamo-hypothalamic domain includes ventral thalamus (VT), posterior entodpeduncular area (PEP), hypothalamic cell cord (HCC), suprachiasmatic area (SCH).
TS20: thalamus	Present	Regionally restricted	1N	Domain includes regions of ventral thalamus (VT), but not the dorsalmost region of the VT.
TS20: hypothalamus	Present	Regionally restricted	1N	Specifically delineates regions of hypothalamic cell cord (HCC).

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	1P

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2B	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: telencephalon	Present	Regionally restricted	2B	Telencephalic domain includes septum (SE), diagonal band (DB), anterior entopeduncular area (AEP), anterior preooptic area (POA).
TS20: lateral ganglionic eminence	Present		2B
TS20: medial ganglionic eminence	Present		2B

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2E

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2H	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: telencephalon	Present	Regionally restricted	2H	Telencephalic domain includes septum (SE), diagonal band (DB), anterior entopeduncular area (AEP), anterior preooptic area (POA).
TS20: diencephalon	Present	Regionally restricted	2H	Thalamo-hypothalamic domain includes ventral thalamus (VT), posterior entodpeduncular area (PEP), hypothalamic cell cord (HCC), suprachiasmatic area (SCH).
TS20: thalamus	Present	Regionally restricted	2H	Domain includes regions of ventral thalamus (VT), but not the dorsalmost region of the VT.
TS20: lateral ganglionic eminence	Present		2H
TS20: medial ganglionic eminence	Present		2H

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2K

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2N	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: diencephalon	Present	Regionally restricted	2N	Thalamo-hypothalamic domain includes ventral thalamus (VT), posterior entodpeduncular area (PEP), hypothalamic cell cord (HCC), suprachiasmatic area (SCH).
TS20: thalamus	Present	Regionally restricted	2N	Domain includes regions of ventral thalamus (VT), but not the dorsalmost region of the VT.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2Q

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2T	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: diencephalon	Present	Regionally restricted	2T	Thalamo-hypothalamic domain includes ventral thalamus (VT), posterior entodpeduncular area (PEP), hypothalamic cell cord (HCC), suprachiasmatic area (SCH).
TS20: thalamus	Present	Regionally restricted	2T	Domain includes regions of ventral thalamus (VT), but not the dorsalmost region of the VT.
TS20: hypothalamus	Present	Regionally restricted	2T	Specifically delineates regions of hypothalamic cell cord (HCC).

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	2W

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	3B	Expression a subset of cells in the paraventricular nucleus (PA) and a stream of cells forming a part of the ventral lateral geniculate body (VLG).
TS22: hypothalamus	Present	Regionally restricted	3B	Expression in the hypothalamic anterodorsal nucleus.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	3D	Expression a subset of cells in the paraventricular nucleus (PA) and a stream of cells forming a part of the ventral lateral geniculate body (VLG).

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	3F
TS22: telencephalon	Present	Regionally restricted	3F	Authors report that abnormal expression in cortical areas has further expanded.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	3H
TS22: diencephalon	Present	Regionally restricted	3H	Extensive labelling only in lateral diencephalic regions.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	4B	Expression a subset of cells in the paraventricular nucleus (PA) and a stream of cells forming a part of the ventral lateral geniculate body (VLG).

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	4D	Expression a subset of cells in the paraventricular nucleus (PA) and a stream of cells forming a part of the ventral lateral geniculate body (VLG).
TS22: thalamus	Present	Regionally restricted	4D	Expression in the central part of the ventral thalamus (zone incerta, ZI; reticular thalamic nuclei, RT).

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	4F
TS22: telencephalon	Present	Regionally restricted	4F	Authors report that abnormal expression in cortical areas has further expanded.
TS22: diencephalon	Present	Regionally restricted	4F	Extensive labelling only in lateral diencephalic regions.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	4H
TS22: telencephalon	Present	Regionally restricted	4H	Authors report that abnormal expression in cortical areas has further expanded.
TS22: diencephalon	Present	Regionally restricted	4H	Extensive labelling only in lateral diencephalic regions.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	5B	Diffuse signal in rostral zona incerta and reticular nucleus; few cells in paraventricular nucleus. Abundant in ventral lateral geniculate body, striatal and pallidal subventricular zone; sharp boundary at level of caudate-putamen.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	5D	Diffuse signal in rostral zona incerta and reticular nucleus; few cells in paraventricular nucleus. Abundant in ventral lateral geniculate body, striatal and pallidal subventricular zone; sharp boundary at level of caudate-putamen.
TS26: telencephalon	Weak	Regionally restricted	5D	Faint signal in ventricular zone and subventricular zone of dorsal telencephalon.
TS26: hypothalamus	Present	Regionally restricted	5D	Expression within anterodorsal and mediodorsal hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	5F	Diffuse signal in rostral zona incerta and reticular nucleus; few cells in paraventricular nucleus. Abundant in ventral lateral geniculate body, striatal and pallidal subventricular zone; sharp boundary at level of caudate-putamen.
TS26: telencephalon	Weak	Regionally restricted	5F	Faint signal in ventricular zone and subventricular zone of dorsal telencephalon.
TS26: hypothalamus	Present	Regionally restricted	5F

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	5H

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	5J

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	5L

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	6A	Expression in two large domains. Region between domains (see below). ('optoeminential zone') does not show expression, except for small subdomain within the supraoptic paraventricular (SPV) area.
TS20: telencephalon	Present	Regionally restricted	6A	Telencephalic domain includes septum (SE), diagonal band (DB), anterior entopeduncular area (AEP), anterior preooptic area (POA).
TS20: lateral ganglionic eminence	Present		6A
TS20: medial ganglionic eminence	Present		6A

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	6B

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	6C	Expression a subset of cells in the paraventricular nucleus (PA) and a stream of cells forming a part of the ventral lateral geniculate body (VLG).

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	6D
TS22: telencephalon	Present	Regionally restricted	6D	Authors report that abnormal expression in cortical areas has further expanded.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	6F	Expression a subset of cells in the paraventricular nucleus (PA) and a stream of cells forming a part of the ventral lateral geniculate body (VLG).

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	6H
TS22: telencephalon	Present	Regionally restricted	6H	Authors report that abnormal expression in cortical areas has further expanded.