Gene Expression Data
Assay Details
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Assay
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Reference:
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J:69855
Martinez-Morales JR, et al., Otx genes are required for tissue specification in the developing eye. Development. 2001 Jun;128(11):2019-30
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2137667
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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anti-Isl1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
8G:
embryonic day 17.5
(more  )
Specimen
8G:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: neural retina |
Present |
Single cells |
8G
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Expression is in RGC and Amacrine cells.
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Specimen
8H,I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx1tm1Asim/Otx1tm1Asim
Otx2tm1Pas/Otx2+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: neural retina |
Present |
Single cells |
8I
,
8H
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In mutant embryos, expression is found in the ectopic retina on the scleral side.
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Specimen
9G:
embryonic day 18.5
(more )
Specimen
9G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neural retina |
Present |
Single cells |
9G
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Expression is in RGC and amacrine cells.
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Specimen
9H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx1tm1Asim/Otx1tm1Asim
Otx2tm1Pas/Otx2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: neural retina |
Present |
Single cells |
9H
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Fewer cells label in mutant embryos.
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Analysis Tools
