Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56466
Brohmann H, et al., The role of Lbx1 in migration of muscle precursor cells. Development. 2000 Jan;127(2):437-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2136978
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe12
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
Not shown:
embryonic day 9.75; Lbx1tm1Cbm/Lbx1 +
(more  )
Specimen
Not shown:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: 1st branchial arch mesenchyme |
Present |
Regionally restricted |
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Expression is present in hypaxial muscle precursors that form the hypoglossal stream. They migrate toward the floor of the first branchial arch (site of the anlage of the tongue).
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1tm1Cbm
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Age:
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embryonic day 9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: 1st branchial arch mesenchyme |
Present |
Regionally restricted |
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Expression is present in hypaxial muscle precursors that form the hypoglossal stream. They migrate toward the floor of the first branchial arch, but in the mutants the cells have not migrated as far as in wild type.
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Specimen
3A:
embryonic day 9.75; Lbx1tm1Cbm/Lbx1 +
(more )
Specimen
3A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud mesenchyme |
Present |
Regionally restricted |
3A
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Expression in the migrating muscle precursor cells that had delaminated from the lateral dermomyotomal lip and moved into the forelimb. They are distributed along the entire proximal forelimb.
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| TS15: trunk dermomyotome |
Present |
Regionally restricted |
3A
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Expression in the migrating muscle precursor cells that had delaminated from the lateral dermomyotomal lip and moved into the forelimb. They are distributed along the entire proximal forelimb.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1tm1Cbm
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Age:
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embryonic day 9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
3B
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The muscle presursor cells moved from the lip of the dermomyotome in a ventral direction but did not enter the limb bud at this stage.
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| TS15: forelimb bud |
Absent |
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3B
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The muscle presursor cells moved from the lip of the dermomyotome in a ventral direction but did not enter the limb bud at this stage.
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Analysis Tools
