Gene Expression Data
Assay Details
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Assay
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Reference:
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J:48569
Grove EA, et al., The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development. 1998 Jun;125(12):2315-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2136860
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Gene symbol:
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Tubb3
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Gene name:
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tubulin, beta 3 class III
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Probe:
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clone p82.2
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Probe preparation:
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Antisense RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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One- and two-color in situ hybridization was performed using digoxygenin- and/or fluorescein-labeled probes, detected with alkaline phosphatase-conjugated anti-digoxigenin or anti-fluorescein antibodies. Color reactions were carried out using different alkaline phosphatase substrates, resulting in different colored products as indicated in some of the figure legends.
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Results
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Specimen
2F:
embryonic day 12.5
(more  )
Specimen
2F:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
2F
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Expression in the cortical cells close to the pial surface, representing a continuation of the developing preplate into the hem.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Weak |
Regionally restricted |
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Expression is weaker in the cortical hem than in the adjacent cortex.
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Specimen
7E:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Strong |
Regionally restricted |
7E
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In brains of non-exencephalic homozygous mutant mice, strong expression in cerebral cortex, specifically in differentiating neurons that have formed a preplate.
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Specimen
5A:
embryonic day 14.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
5A
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Expression marks a curving line of the developing hippocampal pyramidal cell layers.
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Analysis Tools