Probe:	Tgfb3 probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: palatal shelf	Absent

Structure	Level	Pattern	Image	Note
TS20: palatal shelf	Absent

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Present	Regionally restricted	1G , 1H	Expression in region of future medial epithelium; more extensive in vertical epithelium facing tongue; stops abruptly on the oral side. No hybridization within hyperplastic nodules in medial edge epithelia.
TS21: nasal cavity epithelium	Strong	Regionally restricted	1G	High level of expression in the most anterior region of the oro-nasal cavity, the epithelium of the nasal septum.
TS21: nasal septum epithelium	Strong	Regionally restricted	1G	High level of expression in the most anterior region of the oro-nasal cavity, the epithelium of the nasal septum.
TS21: palatal shelf mesenchyme	Absent		1G	No expression in the mesenchyme immediately adjacent to the medial edge epithelium.

Structure	Level	Pattern	Image	Note
TS21: mandible mesenchyme	Weak			Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS21: maxilla	Weak			Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS21: tongue mesenchyme	Weak			Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	2F	Increased expression in medial edge epithelium.
TS22: nasal septum epithelium	Present	Regionally restricted	2F	Striking hybridization in the epithelium of the oral aspect of the prefusion anterior nasal septum.
TS22: mandible	Weak	Regionally restricted	2F	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: maxilla	Weak	Regionally restricted	2F	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: tongue mesenchyme	Weak	Regionally restricted	2F	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: palatal shelf mesenchyme	Absent		2F	No expression in the mesenchyme immediately adjacent to the medial edge epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	2H , 2G	Increased expression in medial edge epithelium.
TS22: nasal septum epithelium	Present	Regionally restricted	2H , 2G	Striking hybridization in the epithelium of the oral aspect of the prefusion anterior nasal septum.
TS22: mandible	Weak	Regionally restricted	2G	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: maxilla	Weak	Regionally restricted	2G	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: tongue mesenchyme	Weak	Regionally restricted	2G	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: palatal shelf mesenchyme	Absent		2G	No expression in the mesenchyme immediately adjacent to the medial edge epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	3D	Medial edge epithelia fuse to form the epithelial seam; expression localised to epithelial cells of the seam, only where the seam is intact.
TS22: mandible	Weak	Regionally restricted	3D	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: maxilla	Weak	Regionally restricted	3D	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: tongue mesenchyme	Weak	Regionally restricted	3D	Low level of hybridization to the mesenchyme of the tongue, mandible and upper regions of the maxillary processes.
TS22: palatal shelf mesenchyme	Absent		3D	No expression in the mesenchyme immediately adjacent to the medial edge epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	3H , 3G	Medial edge epithelia fuse to form the epithelial seam; expression localised to epithelial cells of the seam, only where the seam is intact.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf mesenchyme	Present	Regionally restricted	4D	Hybridization in the midline mesenchyme of the anterior secondary palate.
TS23: nasal septum mesenchyme	Present	Regionally restricted	4D	Hybridization in the perichondrium of the nasal septum.