Gene Expression Data
Assay Details
Assay
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Reference:
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J:32953
Fitzpatrick DR, et al., Differential expression of TGF beta isoforms in murine palatogenesis. Development. 1990 Jul;109(3):585-95
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2136298
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Gene symbol:
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Tgfb2
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Gene name:
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transforming growth factor, beta 2
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Probe:
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Tgfb2 probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
not shown:
embryonic day 11.5
(more )
Note: very early palatal shelves
Specimen
not shown:
(close )
Genetic Background:
|
(Parkes x NIH)F1
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
very early palatal shelves
|
Specimen
not shown:
embryonic day 12.5
(more )
Note: early palatal shelves
Specimen
not shown:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
early palatal shelves
|
Specimen
1D:
embryonic day 13.5
(more )
Note: late vertical palatal shelves
Specimen
1D:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
late vertical palatal shelves
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: palatal shelf epithelium |
Strong |
Regionally restricted |
1D
|
In some sections, high levels of expression in epithelial cells within hyperplastic nodules in medial edge epithelium.
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Specimen
1F:
embryonic day 13.5
(more )
Note: late vertical palatal shelves
Specimen
1F:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
late vertical palatal shelves
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: palatal shelf epithelium |
Strong |
Regionally restricted |
1F
|
In some sections, high levels of expression in epithelial cells within hyperplastic nodules in medial edge epithelium.
|
Specimen
not shown:
embryonic day 13.5
(more )
Note: late vertical palatal shelves
Specimen
not shown:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
late vertical palatal shelves
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: olfactory epithelium |
Present |
Regionally restricted |
|
Expression in olfactory epithelium of the nasal process
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Specimen
2E:
embryonic day 14.0
(more )
Note: horizontal palatal shelves
Specimen
2E:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
horizontal palatal shelves
|
Specimen
3C:
embryonic day 14.5
(more )
Note: fusing palate
Specimen
3C:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
fusing palate
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: palatal shelf mesenchyme |
Present |
Regionally restricted |
3C
|
Transcript levels increase; easily seen in mesenchyme on either side of the epithelial seam; asymmetric distribution with higher concentration toward nasal side of palate.
|
Specimen
3E,F:
embryonic day 14.5
(more )
Note: fusing palate
Specimen
3E,F:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
fusing palate
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: palatal shelf mesenchyme |
Present |
Regionally restricted |
3E
,
3F
|
Transcript levels increase; easily seen in mesenchyme on either side of the epithelial seam; asymmetric distribution with higher concentration toward nasal side of palate.
|
TS22: palatal shelf epithelium |
Absent |
|
3F
,
3E
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No expression in the epithelial seam itself.
|
Specimen
4C:
embryonic day 15.5
(more )
Note: maturing palate
Specimen
4C:
(close )
Genetic Background:
|
(Parkes x NIH)F1
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
maturing palate
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: palatal shelf mesenchyme |
Present |
Regionally restricted |
4C
|
Diffuse expression in mesenchyme around the midline of the palate.
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