Antibody:	281-2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Tissues were fixed in Carnoy's fluid (ethanol, chloroform, glacial acetic acid). Authors report identical changes in expression were seen with Bouin's and Sainte Marie's fixatives, as well as after pre-treating sections with chondroitinase ABC.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		1A	Staining at the basolateral surfaces of the single layer of surface ectoderm cells and over the entire surfaces of neuroectoderm cells.
TS13: surface ectoderm	Present		1A	Staining at the basolateral surfaces of the single layer of surface ectoderm cells and over the entire surfaces of neuroectoderm cells.
TS13: head mesenchyme	Weak	Regionally restricted	1A	Minimal/faint stain on intervening cranial mesenchyme cells lateral to the optic vesicle.

Structure	Level	Pattern	Image	Note
TS13: sensory organ system	Absent			Authors report that cells forming the nasal placodes lose stain.
TS13: otic placode epithelium	Absent			Otic placodes lose their stain when they elongate. (Re-expression in otic placode derivatives, such as semi-circular canal epithelium.)

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present	Regionally restricted	1B	Surface ectoderm cells adjacent to the forming placode retain stain.
TS15: nervous system	Absent		1B	Neuroectoderm no longer stains.
TS15: lens placode	Weak		1B	Cells of the forming lens placode show markedly reduced stain.
TS15: eye mesenchyme	Strong	Regionally restricted	1B	Mesenchyme cells adjacent to the lens placode become intensely positive.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Absent		1C
TS17: optic cup	Absent		1C
TS17: head mesenchyme	Present		1C	Surface ectoderm cells and adjacent mesenchyme cells retain stain at their surfaces.
TS17: surface ectoderm	Present		1C	Surface ectoderm cells and adjacent mesenchyme cells retain stain at their surfaces.

Structure	Level	Pattern	Image	Note
TS17: nasal pit	Absent		2A	Olfactory or nasal pits persist in not showing stain.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch maxillary component ectoderm	Present			Maxillary process shows well-stained surface ectoderm cells.
TS17: 1st branchial arch maxillary component mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Absent		1D	Mesenchyme cells and ectoderm cells in contact with the lens vesicle lose stain.
TS19: surface ectoderm	Present	Regionally restricted	1D	Surface ectoderm cells in the region of the lens vesicle and in the nasal region stain.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Present	Regionally restricted	2B	Surface ectoderm cells in the region of the lens vesicle and in the nasal region stain.
TS19: nasal pit epithelium	Absent		2B	Ectoderm forming the nasal pit does not stain.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Present	Regionally restricted	3A	Surface ectoderm cells in the region of the lens vesicle and in the nasal region stain.
TS19: maxillary process	Weak	Regionally restricted	3A	In the maxillary region, stain is reduced in epithelium invaginating to form vibrissal rudiments.
TS19: maxillary process mesenchyme	Strong	Regionally restricted	3A	Underlying condensed mesenchyme stains intensely.

Structure	Level	Pattern	Image	Note
TS20: maxillary process epithelium	Absent		3B	Stain is lost from invaginating epithelial cells.
TS20: maxillary process mesenchyme	Present	Regionally restricted	3B	Stain persists in underlying condensed mesenchyme.
TS20: skin	Present	Regionally restricted	3B	Ectoderm adjacent to vibrissal rudiments in the maxillary region remains stained.

Structure	Level	Pattern	Image	Note
TS23: eyelid	Strong	Regionally restricted	1E	Stratified epidermis covering the eyelid show abundant stain.
TS23: conjunctival sac	Strong	Regionally restricted	1E	Abundant stain in palpebral and bulbar conjunctivae.
TS23: cornea	Present	Regionally restricted	1E	Corneal endothelium does not stain.
TS23: cornea epithelium	Strong		1E
TS23: lens	Absent		1E	Lens epithelium and lens fiber cells do not show stain.
TS23: lens fiber	Absent		1E	Lens epithelium and lens fiber cells do not show stain.
TS23: eye	Strong	Regionally restricted	1E	Abundant staining in the periderm.

Structure	Level	Pattern	Image	Note
TS23: nasal cavity epithelium	Present	Regionally restricted	2C	Re-expression in the epithelium constituting the nasal passages; basal cell layer stains at its apical and lateral surfaces; more superficial cells in the distal region show stain over their entire surface
TS23: nasopharynx epithelium	Present	Regionally restricted	2C	Epithelial cells in the nasal passayes near the nasopharynx are devoid of stain.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted	3C	In the maxillary region, stain surrounding the stratified cells of the epidermis.
TS23: vibrissa epidermal component	Present	Regionally restricted	3C	During vibrissal development, the external root sheath re-expressed stain, but stain is lost from elongated cells at the neck of the hair shaft.
TS23: vibrissa dermal component	Absent		3C	During vibrissal development, stain is absent in the mesenchyme, including the hair papilla.