Probe:	CRBPI cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1A CRBP	Well-defined labelling in specific regions.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1B CRBP	Well-defined labelling in specific regions.
TS11: primitive streak	Present	Regionally restricted	1B CRBP	Highest expression in epiblast and primary mesenchyme of the primitive streak.
TS11: embryo mesenchyme	Present	Regionally restricted	1B CRBP	Expression in the most recently formed mesenchyme adjacent to the primitive streak.
TS11: neural ectoderm	Present	Regionally restricted	1B CRBP	Some labelling of basal and apical surfaces of more rostral neural epithelium.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	2A CRBP	Present at the basal and apical neuroepithelial cell surfaces.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		2B CRBP	Labelling in epiblast and mesenchyme of the primitive streak.
TS12: future hindbrain neural ectoderm	Present	Regionally restricted	2B CRBP	At rostral hindbrain level, present at basal and apical neuroepithelial cells surfaces. Strongest labelling in caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		3_CRBP	Labelling in epiblast and mesenchyme of the primitive streak.
TS12: future midbrain	Present	Regionally restricted	3_CRBP	Present at the basal and apical neuroepithelial cell surfaces.
TS12: future hindbrain neural ectoderm	Present	Regionally restricted	3_CRBP	At rostral hindbrain level, present at basal and apical neuroepithelial cells surfaces. Strongest labelling in caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	5A CRBP	rostral limit of expression was not a clear boundary, but a gradual diminution from caudal to rostral levels.
TS13: future prosencephalon	Present		5A CRBP	Signal was very low in the forebrain.
TS13: head mesenchyme	Present	Regionally restricted	5A CRBP	Labelling was strongest in areas assumed to be occupied by neural crest-derived cells.
TS13: extraembryonic component	Present	Regionally restricted	5A CRBP	Expression in the endodermal layer of the yolk sac.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	5B CRBP	rostral limit of expression was not a clear boundary, but a gradual diminution from caudal to rostral levels.
TS13: head mesenchyme	Present	Regionally restricted	5B CRBP	Labelling was strongest in areas assumed to be occupied by neural crest-derived cells.
TS13: extraembryonic component	Present	Regionally restricted	5B CRBP	Expression in the endodermal layer of the yolk sac.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	5C CRBP	rostral limit of expression was not a clear boundary, but a gradual diminution from caudal to rostral levels.
TS13: trunk unsegmented mesenchyme	Present		5C CRBP
TS13: primitive streak	Strong	Regionally restricted	5C CRBP	Strong signal in the primitive streak itself.
TS13: extraembryonic component	Present	Regionally restricted	5C CRBP	Expression in the endodermal layer of the yolk sac.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	5D CRBP	Strongest signal apically and basally.
TS13: future brain	Present	Regionally restricted	5D CRBP	rostral limit of expression was not a clear boundary, but a gradual diminution from caudal to rostral levels.
TS13: trunk unsegmented mesenchyme	Present		5D CRBP
TS13: future spinal cord neural fold	Present	Regionally restricted	5D CRBP	Strong signal just rostral to the primitive streak.
TS13: extraembryonic component	Present	Regionally restricted	5D CRBP	Expression in the endodermal layer of the yolk sac.

Structure	Level	Pattern	Image	Note
TS13: trunk somite	Present	Regionally restricted		Transcripts in the youngest somites.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	6A CRBP	Transcripts in the medial half of the most rostral hindbrain, except for midline supranotochordal cells. At mandibular arch level, restricted to a small band just dorsal to the supranotochordal cells.
TS14: tail bud	Present	Regionally restricted	6A CRBP	Authors report high levels of transcripts in tail bud mesoderm and epiblast.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	6B CRBP	Transcripts in the medial half of the most rostral hindbrain, except for midline supranotochordal cells. At mandibular arch level, restricted to a small band just dorsal to the supranotochordal cells.
TS14: trunk somite	Present	Regionally restricted	6B CRBP	High levels of transcripts in the youngest somites.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	6C CRBP	As far as the posterior neuropore, only the basal and apical surface regions were labelled.
TS14: gut	Present	Regionally restricted	6C CRBP	Transcripts in gut endoderm.

Structure	Level	Pattern	Image	Note
TS14: otic placode epithelium	Present	Regionally restricted		Only the basal and apical surface regions were labelled.
TS14: intermediate mesenchyme	Present
TS14: mesenchyme derived from lateral plate	Present
TS14: branchial arch	Present	Regionally restricted		Transcripts in frontonasal and phyaryngeal arch mesenchyme, and in mesenchyme adjacent to the heart.
TS14: head mesenchyme	Present	Regionally restricted		Transcripts in frontonasal and phyaryngeal arch mesenchyme, and in mesenchyme adjacent to the heart.
TS14: trunk mesenchyme	Present	Regionally restricted		Transcripts in frontonasal and phyaryngeal arch mesenchyme, and in mesenchyme adjacent to the heart.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Regionally restricted	8A CRBP	Authors state that the pattern of expression was not very different from that seen previously.
TS14: head mesenchyme derived from neural crest	Present		8A CRBP	Higher levels of transcripts in neural crest-derived cells.
TS14: mesenchyme derived from head mesoderm	Present		8A CRBP	Higher levels of transcripts in neural crest-derived cells.
TS14: head mesenchyme	Present	Regionally restricted	8A CRBP	Expression in ring of frontonasal mesenchyme around the forebrain vesicle.
TS14: 1st branchial arch mandibular component mesenchyme	Present		8A CRBP
TS14: otic pit epithelium	Present		8A CRBP	Strongest signal at this stage was on the basal side of the otic pit epithelium.
TS14: extraembryonic component	Present	Regionally restricted	8A CRBP	Strong expression in the yolk sac endoderm.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Regionally restricted	8B CRBP	Authors state that the pattern of expression was not very different from that seen previously.
TS14: head mesenchyme derived from neural crest	Present		8B CRBP	Higher levels of transcripts in neural crest-derived cells.
TS14: mesenchyme derived from head mesoderm	Present		8B CRBP	Higher levels of transcripts in neural crest-derived cells.
TS14: otic pit epithelium	Present		8B CRBP	Strongest signal at this stage was on the basal side of the otic pit epithelium.
TS14: extraembryonic component	Present	Regionally restricted	8B CRBP	Strong expression in the yolk sac endoderm.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present			Authors report that labelling was present in the first seven rhombomeres.
TS14: rhombomere 2	Present			Authors report that labelling was present in the first seven rhombomeres.
TS14: rhombomere 3	Present			Authors report that labelling was present in the first seven rhombomeres.
TS14: rhombomere 4	Present			Authors report that labelling was present in the first seven rhombomeres.
TS14: rhombomere 5	Present			Authors report that labelling was present in the first seven rhombomeres.
TS14: rhombomere 6	Present			Authors report that labelling was present in the first seven rhombomeres.
TS14: rhombomere 7	Present			Authors report that labelling was present in the first seven rhombomeres.
TS14: foregut	Present	Regionally restricted		Labelling present in gut endoderm.
TS14: gut	Present	Regionally restricted		Labelling present in gut endoderm.
TS14: trunk somite	Present	Regionally restricted		Transcripts in the most recently formed somites.