Probe:	RAR-alpha-cDNA4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Ambiguous			Authors state that signal was diffuse, indicating either very low levels of expression or background of hybridization.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted		Authors report slightly higher signal in epiblast and mesoderm of primitive streak than in the rest of the embryo.
TS12: primitive streak	Present

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		5A RAR-alpha	Transcripts in the neural epithelium of the forebrain.
TS13: head mesenchyme derived from neural crest	Present		5A RAR-alpha	Expression in neural crest cells of the frontonasal and first pharyngeal arch regions.
TS13: migrating neural crest	Weak		5A RAR-alpha

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme derived from neural crest	Present		5B RAR-alpha	Expression in neural crest cells of the frontonasal and first pharyngeal arch regions.
TS13: migrating neural crest	Weak		5B RAR-alpha

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	5C RAR-alpha	Transcripts at the level caudal to the pre-otic sulcus; strongest in lateral neural epithelium from which neural crest cells were emigrating, and in crest cells themselves.
TS13: migrating neural crest	Present		5C RAR-alpha

Structure	Level	Pattern	Image	Note
TS14: embryo	Present		6A RAR-alpha	Transcripts were almost ubiquitously expressed.
TS14: branchial arch mesenchyme	Weak	Regionally restricted	6A RAR-alpha	Lower intensity in frontonasal and pharyngeal arch mesenchyme.
TS14: head mesenchyme	Weak	Regionally restricted	6A RAR-alpha	Lower intensity in frontonasal and pharyngeal arch mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present		6B RAR-alpha	Transcripts were almost ubiquitously expressed.
TS14: migrating neural crest	Present	Regionally restricted	6B RAR-alpha	Presumed neural crest cells were most strongly labelled in hindbrain levels from which neural crest cells were emigrating.
TS14: branchial arch mesenchyme	Present	Regionally restricted	6B RAR-alpha	Lower intensity in frontonasal and pharyngeal arch mesenchyme.
TS14: head mesenchyme	Present	Regionally restricted	6B RAR-alpha	Lower intensity in frontonasal and pharyngeal arch mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present		6C RAR-alpha	Transcripts were almost ubiquitously expressed.
TS14: mesenchyme derived from head mesoderm	Ambiguous		6C RAR-alpha	Authors report little or no expression in cranial primary mesenchyme or in occipital somites.
TS14: head somite	Ambiguous		6C RAR-alpha	Authors report little or no expression in cranial primary mesenchyme or in occipital somites.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Regionally restricted	8A RAR-alpha	Authors state that the pattern of expression was not very different from that seen previoiusly.
TS14: head mesenchyme derived from neural crest	Present		8A RAR-alpha	Higher levels of transcripts in neural crest-derived cells.
TS14: mesenchyme derived from head mesoderm	Present		8A RAR-alpha	Higher levels of transcripts in neural crest-derived cells.
TS14: 1st branchial arch mandibular component	Present	Regionally restricted	8A RAR-alpha	Expression in a ring of frontonasal mesenchyme around the forebrain vesicle.
TS14: neural tube	Present	Regionally restricted	8A RAR-alpha	Higher levels of trancripts in outer cells of neural tube (ie. the newly forming mantle layer, containing neuroblasts).
TS14: heart	Absent		8A RAR-alpha
TS14: limb	Present	Regionally restricted	8A RAR-alpha	Transcripts in all mesodermal components of the limb.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted		Anterior limit of expression at the rhombomere 3/4 boundary.
TS14: trunk mesenchyme	Present	Regionally restricted		Transcripts in all mesodermal components of the trunk, except for the heart.