Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49150
Yang Y, et al., Role of laminin polymerization at the epithelial mesenchymal interface in bronchial myogenesis. Development. 1998 Jul;125(14):2621-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1934362
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Gene symbol:
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Acta2
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Gene name:
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actin alpha 2, smooth muscle, aorta
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Antibody:
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anti SM a-actin
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1. B.:
embryonic day 12.5
(more  )
Note: Specimens were fixed in absolute alcohol.
Specimen
1. B.:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Specimens were fixed in absolute alcohol.
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Specimen
1. D.:
embryonic day 14.5
(more )
Note: Specimens were fixed in absolute alcohol.
Specimen
1. D.:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Specimens were fixed in absolute alcohol.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: main bronchus mesenchyme |
Present |
Regionally restricted |
1D
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Expression is detected in one or two layers of mesenchymal cells surrounding the main bronchi; intensity of expression increased compared to E12.5.
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| TS22: lobar bronchus mesenchyme |
Present |
Regionally restricted |
1D
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Expression extended into the proximal portions of the second order bronchi and walls of the main blood vessels.
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| TS22: main bronchus vascular element |
Present |
Regionally restricted |
1D
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Expression extended into the proximal portions of the second order bronchi and walls of the main blood vessels.
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Specimen
E11.5:
embryonic day 11.5
(more )
Note: Specimens were fixed in absolute alcohol.
Specimen
E11.5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Specimens were fixed in absolute alcohol.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trachea epithelium |
Present |
Regionally restricted |
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Expression is detected in mesenchymal cells around the tracheal epithelium.
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| TS19: trachea mesenchyme |
Present |
Regionally restricted |
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Expression is detected in mesenchymal cells around the tracheal epithelium.
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Analysis Tools