Antibody:	anti-NCAM
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Strong	Regionally restricted	2F	Immunoreactivity was strong in the caudal neural tube and neural plate
TS13: neural tube	Strong	Regionally restricted	2F	Immunoreactivity was strong in the caudal neural tube and neural plate
TS13: future brain	Weak	Uniform	2F	Very weak, diffuse labelling in the head folds. Authors interpret these low, uniform levels of labelling as nonspecific background staining.
TS13: head mesenchyme	Absent		2F
TS13: trunk somite	Present		2F

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural plate	Strong	Regionally restricted	2G	Immunoreactivity was strong in the caudal neural tube and neural plate.
TS14: neural tube	Strong	Regionally restricted	2G	Immunoreactivity was strong in the caudal neural tube and neural plate.
TS14: future brain	Weak	Uniform	2G	Very weak, diffuse labelling in the head folds. Authors interpret these low, uniform levels of labelling as nonspecific background staining.
TS14: head mesenchyme	Absent		2G
TS14: trunk somite	Present		2G

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural plate	Strong	Regionally restricted	2H	Immunoreactivity was strong in the caudal neural tube and neural plate.
TS14: neural tube	Strong	Regionally restricted	2H	Immunoreactivity was strong in the caudal neural tube and neural plate.
TS14: future brain	Weak	Uniform	2H	Very weak, diffuse labelling in the head folds. Authors interpret these low, uniform levels of labelling as nonspecific background staining.
TS14: head mesenchyme	Absent		2H
TS14: trunk somite	Present		2H