Gene Expression Data
Assay Details
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Assay
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Reference:
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J:4658
Bally-Cuif L, et al., The mouse NCAM gene displays a biphasic expression pattern during neural tube development. Development. 1993 Feb;117(2):543-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1934358
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Probe:
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DW3-le
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A,2B:
embryonic day 8.5
(more  )
Note: 8 somites.
Specimen
2A,2B:
(close  )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: trunk somite |
Present |
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2A;B
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Expression was detected in all somites.
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| TS12: future spinal cord |
Present |
Regionally restricted |
2A;B
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Expression was detected in the caudal neurectoderm.
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| TS12: future spinal cord neural fold |
Present |
Regionally restricted |
2A;B
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Expression signal was most intense in the neural folds around the rostral part of the posterior neuropore, weaker around its caudal part.
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| TS12: neural tube |
Present |
Regionally restricted |
2A;B
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Signal was weaker rostrally in the recently closed neural tube.
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| TS12: future hindbrain |
Present |
Regionally restricted |
2A;B
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Signal in the neural tube extended up to a rostral limit beyond the first somite, approximately halfway into the hindbrain, where it faded out.
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| TS12: embryo ectoderm |
Present |
Regionally restricted |
2A;B
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Expression was absent from the headfolds.
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Specimen
2C:
embryonic day 8.5
(more )
Note: 8 somites.
Specimen
2C:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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8 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future spinal cord neural plate |
Present |
Regionally restricted |
2C
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Expression was detected throughout the entire width of the neural plate and tube. Expression was more intense where tube was not yet closed. Signal was stronger near the lumen of the tube.
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| TS12: neural tube |
Present |
Regionally restricted |
2C
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Expression was detected throughout the entire width of the neural plate and tube. Expression was more intense where tube was not yet closed. Signal was stronger near the lumen of the tube.
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| TS12: trunk unsegmented mesenchyme |
Present |
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2C
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Specimen
2D:
embryonic day 8.5
(more )
Note: 8 somites.
Specimen
2D:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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8 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future spinal cord neural plate |
Present |
Regionally restricted |
2D
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Expression was detected throughout the entire width of the neural plate and tube. Expression was more intense where tube was not yet closed. Signal was stronger near the lumen of the tube.
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| TS12: neural tube |
Present |
Regionally restricted |
2D
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Expression was detected throughout the entire width of the neural plate and tube. Expression was more intense where tube was not yet closed. Signal was stronger near the lumen of the tube.
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| TS12: trunk unsegmented mesenchyme |
Present |
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2D
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Specimen
2E:
embryonic day 8.75
(more )
Note: 14 somites.
Specimen
2E:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future spinal cord |
Present |
Regionally restricted |
2E
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Expression in the germinative neuroepithelium has shifted caudally to the level between somites 12 and 14.
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| TS14: trunk somite |
Present |
Regionally restricted |
2E
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Staining of somites changed according to rostrocaudal gradient of development; caudal somites were labelled throughout their width.
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| TS14: trunk dermomyotome |
Present |
Regionally restricted |
2E
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There was progressive concentration in the newly formed dermomyotome rostrally.
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