Gene Expression Data
Assay Details
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Assay
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Reference:
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J:21617
Gunther T, et al., Open brain, a new mouse mutant with severe neural tube defects, shows altered gene expression patterns in the developing spinal cord. Development. 1994 Nov;120(11):3119-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933421
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 10.5; Rab23opb/ Rab23opb
(more  )
Note: The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
Specimen
not shown:
(close  )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The original opb carriers were of a mixed outbred NMRI background. The opb mutant embryos used in this study were derived subsequent to at least 6 crosses of opb mutant males to wild-type C57Bl/6 females.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
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Authors state that Pax3 expression patterns (in the future dermomyotome) revealed that paraxial mesenchyme in the thoracic region becomes properly segmented. However, in dorsal somitic regions, an abnormal, flame-like pattern of expression was observed.
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| TS17: trunk paraxial mesenchyme |
Present |
Regionally restricted |
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Authors state that Pax3 expression patterns (in the future dermomyotome) revealed that paraxial mesenchyme in the thoracic region becomes properly segmented. However, in dorsal somitic regions, an abnormal, flame-like pattern of expression was observed.
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| TS17: trunk somite |
Present |
Regionally restricted |
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Authors state that Pax3 expression patterns (in the future dermomyotome) revealed that paraxial mesenchyme in the thoracic region becomes properly segmented. However, in dorsal somitic regions, an abnormal, flame-like pattern of expression was observed.
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Analysis Tools
