Gene Expression Data
Assay Details
Assay
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Reference:
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J:13164
Faerman A, et al., The expression of the regulatory myosin light chain 2 gene during mouse embryogenesis. Development. 1993 Jul;118(3):919-29
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1932363
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Gene symbol:
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Myog
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Gene name:
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myogenin
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Probe:
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Myog cDNA16
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1C:
embryonic day 9.5
(more )
Note: 24-26 somites.
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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24-26 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
Regionally restricted |
1C
|
Expressed in the myotomal part of the first 15-18 somites, and in the next one to two more posterior (less mature) somites. No expression in most caudal somites.
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Specimen
1F:
embryonic day 9.5
(more )
Note: 24-26 somites.
Specimen
1F:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
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24-26 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
Regionally restricted |
1F
|
Expressed in the myotomal part of the first 15-18 somites, and in the next one to two more posterior (less mature) somites. No expression in most caudal somites.
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Specimen
3D:
embryonic day 10.5
(more )
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: head mesenchyme |
Present |
Regionally restricted |
3D
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Expressed in extramyotomal premuscle cell masses in the cervical region, including the laryngeal premuscle cell mass and the posterior part of the hypoglossal cord.
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TS17: myotome |
Present |
Regionally restricted |
3D
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Expressed in the myotomal part of most somites, both rostral and caudal.
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Specimen
4D:
embryonic day 10.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5B:
embryonic day 10.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: 2nd branchial arch mesenchyme |
Present |
Regionally restricted |
5B
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Transcripts in premuscle cell masses in the pharyngeal region, including the dorsolateral part of the 2nd visceral arch, hypoglossal cords, and laryngeal muscle anlage.
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TS17: head mesenchyme |
Present |
Regionally restricted |
5B
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Transcripts in premuscle cell masses in the pharyngeal region, including the dorsolateral part of the 2nd visceral arch, hypoglossal cords, and laryngeal muscle anlage.
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Specimen
6C:
embryonic day 11.5
(more )
Note: There are discrepancies in the figure legend as to which gene expression is of Fig. 6D or 6C.
Specimen
6C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Note:
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There are discrepancies in the figure legend as to which gene expression is of Fig. 6D or 6C.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk myotome |
Strong |
Regionally restricted |
6C
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Strong hybridization in diaphragmal premuscle masses at the level of the forelimb buds.
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Specimen
7B:
embryonic day 11.5
(more )
Specimen
7B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk myotome |
Strong |
Regionally restricted |
7B
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Strong hybridization in mytomes above the limb buds.
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TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
7B
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Expressed in ventral and dorsal premuscle cell masses in posterior regions of the limb bud.
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Specimen
7F:
embryonic day 11.5
(more )
Specimen
7F:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk myotome |
Strong |
Regionally restricted |
7F
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Strong hybridization in mytomes above the limb buds.
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TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
7F
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Expressed in few cells in the dorsal premuscle cell masses in anterior regions of the limb bud.
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Specimen
8B/F:
embryonic day 12.5
(more )
Specimen
8B/F:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head mesenchyme |
Present |
Regionally restricted |
8B
,
8F
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In the most superficial muscle (platysma) anlage in the pharyngeal region, only expressed in few cells.
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Specimen
not shown:
embryonic day 9.0
(more )
Note: 15 somites.
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.0
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Note:
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15 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: somite |
Present |
Regionally restricted |
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Expressed in rostral somites.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: perioptic mesenchyme |
Present |
Regionally restricted |
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Expressed in the extraocular muscle rudiments.
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TS19: forelimb bud mesenchyme |
Present |
|
|
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TS19: 1st branchial arch |
Present |
Regionally restricted |
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Expressed in muscle-forming regions delineated in previous stages, including parts of the 1st and 2nd branchial arches, hypoglossal cords, and laryngeal muscle anlage.
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TS19: 2nd branchial arch mesenchyme |
Present |
Regionally restricted |
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Expressed in muscle-forming regions delineated in previous stages, including parts of the 1st and 2nd branchial arches, hypoglossal cords, and laryngeal muscle anlage.
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TS19: head mesenchyme |
Present |
Regionally restricted |
|
Expressed in muscle-forming regions delineated in previous stages, including parts of the 1st and 2nd branchial arches, hypoglossal cords, and laryngeal muscle anlage.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|