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Gene Expression Data
Assay Details
Assay
Reference: J:19631 McKay IJ, et al., The kreisler mouse: a hindbrain segmentation mutant that lacks two rhombomeres. Development. 1994 Aug;120(8):2199-211
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1932361
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament clone 2H3
Assay notes: Kreisler -/- mutants at dpc 9.5 have an absence of morphological segmentation posterior to the boundary between r3 and r4.
Results
Specimen 4A: embryonic day 9.5; Mafbkr/Mafb+ (more )
Note: Flat-mount
Structure Level Pattern Image Note
TS15: rhombomere 3 Absent 4A
TS15: rhombomere 5 Absent 4A
TS15: future hindbrain Present Regionally restricted 4A Antibody reveals a subset of developing neurons within the hindbrain.
Specimen 4B: embryonic day 9.5; Mafbkr/Mafbkr (more )
Note: Flat-mount
Structure Level Pattern Image Note
TS15: rhombomere 3 Absent 4B
Specimen 4C: embryonic day 10.5; Mafbkr/Mafb+ (more )
Note: Flat-mount
Structure Level Pattern Image Note
TS17: rhombomere 1 Present Regionally restricted 4C Rhombomere boundaries demarcating r1-6.
TS17: rhombomere 2 Present Regionally restricted 4C Rhombomere boundaries demarcating r1-6.
TS17: rhombomere 3 Present Regionally restricted 4C Rhombomere boundaries demarcating r1-6.
TS17: rhombomere 4 Present Regionally restricted 4C Rhombomere boundaries demarcating r1-6.
TS17: rhombomere 5 Present Regionally restricted 4C Rhombomere boundaries demarcating r1-6.
TS17: rhombomere 6 Present Regionally restricted 4C Rhombomere boundaries demarcating r1-6.
Specimen 4D: embryonic day 10.5; Mafbkr/Mafbkr (more )
Note: Flat-mount
Structure Level Pattern Image Note
TS17: rhombomere 1 Present Regionally restricted 4D A reduced number of rhombomere boundaries demarcating only r1-4.
TS17: rhombomere 2 Present Regionally restricted 4D A reduced number of rhombomere boundaries demarcating only r1-4.
TS17: rhombomere 3 Present Regionally restricted 4D A reduced number of rhombomere boundaries demarcating only r1-4.
TS17: rhombomere 4 Present Regionally restricted 4D A reduced number of rhombomere boundaries demarcating only r1-4.
Specimen 4E: embryonic day 10.5; Mafbkr/Mafb+ (more )
Structure Level Pattern Image Note
TS17: facial ganglion Present 4E The pattern reveals the cranial nerves and ganglia.
TS17: glossopharyngeal ganglion Present 4E The pattern reveals the cranial nerves and ganglia.
TS17: trigeminal ganglion Present 4E The pattern reveals the cranial nerves and ganglia.
TS17: vagus ganglion Present 4E The pattern reveals the cranial nerves and ganglia.
Specimen 4F: embryonic day 10.5; Mafbkr/Mafbkr (more )
Structure Level Pattern Image Note
TS17: facial ganglion Present 4F
TS17: trigeminal ganglion Present 4F
TS17: glossopharyngeal ganglion Present 4F An apparent fusion of nerves IX and X.
TS17: vagus ganglion Present 4F An apparent fusion of nerves IX and X.
Specimen 4G: embryonic day 11.5; Mafbkr/Mafb+ (more )
Structure Level Pattern Image Note
TS19: cranial nerve Present 4G Abducens VI nerve and its rostral trajectory.
TS19: facial ganglion Present 4G
TS19: hypoglossal nerve Present 4G
Specimen 4H: embryonic day 11.5; Mafbkr/Mafbkr (more )
Structure Level Pattern Image Note
TS19: cranial nerve Present Regionally restricted 4H Although there is expression is other cranial nerves, there is an absence of any neuronal structure resembling the abducens.
TS19: facial ganglion Present 4H
TS19: hypoglossal nerve Present 4H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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