Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63681
Martinez-Barbera JP, et al., The homeobox gene Hesx1 is required in the anterior neural ectoderm for normal forebrain formation. Dev Biol. 2000 Jul 15;223(2):422-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1931459
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Gene symbol:
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Otx1
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Gene name:
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orthodenticle homeobox 1
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Probe:
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Otx1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6 I:
embryonic day 8.0
(more  )
Note: 7 somites.
Specimen
6 I:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
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6I
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| TS12: future prosencephalon |
Present |
Regionally restricted |
6I
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Expression is in an inverted U-shaped domain in the prosencephalon. This domain abuts the rostromedial aspects of the forebrain, including the incipient optic cups.
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| TS12: future brain |
Present |
Regionally restricted |
6I
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Expression domain in the lateral aspects of the anterior neural plate. Expression was absent in the medial region of the anterior neural plate.
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Specimen
6 J:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Hesx1tm1Icar/Hesx1tm1Icar
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
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6J
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| TS12: future prosencephalon |
Present |
Regionally restricted |
6J
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Expression is absent in a region in the rostral forebrain but expression is normal in other forebrain territories.
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| TS12: future brain |
Present |
Regionally restricted |
6J
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Expression domain in the lateral aspects of the anterior neural plate. The medial region of the anterior neural plate (which normally does not express Otx1) is absent in the embryo, and now expression was seen across the midline.
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Analysis Tools
