Gene Expression Data
Assay Details
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Assay
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Reference:
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J:66592
Liu A, et al., EN and GBX2 play essential roles downstream of FGF8 in patterning the mouse mid/hindbrain region. Development. 2001 Jan;128(2):181-91
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1929102
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 cDNA9
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2. D:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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En1tm1Alj/En1+
En2tm1Alj/En2tm1Alj
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2. D':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
En2tm2Alj/En2tm2Alj
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3. K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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En1tm1Alj/En1+
En2tm1Alj/En2tm1Alj
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 11 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
3K
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Expression is detected in the anterior hindbrain.
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Specimen
3. K':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
En2tm2Alj/En2tm2Alj
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Embryo is at the 11 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
Regionally restricted |
3K'
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Expression is detected in the anterior hindbrain but the area of expression is expanded caudally compared to wild type.
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Analysis Tools
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