Probe:	Hox 2.9 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo mesenchyme	Present	Regionally restricted	3A/B	Expression is detected within the primitive streak in the newly formed mesoderm and overlying neurectoderm.
TS11: neural ectoderm	Present	Regionally restricted	3A/B	Expression is detected within the primitive streak in the newly formed mesoderm and overlying neurectoderm.
TS11: primitive streak	Present	Regionally restricted	3A/B	Expression is detected within the primitive streak in the newly formed mesoderm and overlying neurectoderm.

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Strong	Regionally restricted	4C	Expression is detected in the neurectoderm and mesoderm extending from the posterior of the embryo into the region of the developing hindbrain with a sharp boundary at the preotic sulcus.
TS12: future hindbrain	Strong	Regionally restricted	4C	Expression is detected in the neurectoderm and mesoderm extending from the posterior of the embryo into the region of the developing hindbrain with a sharp boundary at the preotic sulcus.
TS12: future hindbrain neural ectoderm	Strong	Regionally restricted	4C	Expression is detected in the neurectoderm and mesoderm extending from the posterior of the embryo into the region of the developing hindbrain with a sharp boundary at the preotic sulcus.
TS12: neural ectoderm	Strong	Regionally restricted	4C	Expression is detected in the neurectoderm and mesoderm extending from the posterior of the embryo into the region of the developing hindbrain with a sharp boundary at the preotic sulcus.
TS12: pre-otic sulcus	Strong	Regionally restricted	4C	Expression is detected in the neurectoderm and mesoderm extending from the posterior of the embryo into the region of the developing hindbrain with a sharp boundary at the preotic sulcus.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	4G	Expression is restricted to the hindbrain with the anterior boundary of expression at the pre-otic sulcus.
TS13: pre-otic sulcus	Present	Regionally restricted	4G	Expression is restricted to the hindbrain with the anterior boundary of expression at the pre-otic sulcus.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 4	Present		4J
TS14: embryo mesenchyme	Present		4J	Expression is detected in the mesoderm lateral to rhombomere four where the sensory ganglia are condensing.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	5A/B	Expression is detected in neural crest cells migrating from rhombomere 4.
TS14: rhombomere 4	Present	Regionally restricted	5A/B	Expression is detected in neural crest cells migrating from rhombomere 4.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted	5D/E	Expression detected in the lateral plate mesoderm as far as the anterior headfold.
TS14: trunk unsegmented mesenchyme	Present		5D/E
TS14: trunk somite	Weak		5D/E

Structure	Level	Pattern	Image	Note
TS15: gut	Present	Regionally restricted	5G/H	Expression is detected in the gut epithelium at the level of the forelimb bud and the gut associated mesoderm at and below the level of the heart.
TS15: rhombomere 4	Strong		5G/H
TS15: tail unsegmented mesenchyme	Present		5G/H

Structure	Level	Pattern	Image	Note
TS15: gut	Present	Regionally restricted	5J/K	Expression is detected in the gut associated mesoderm at and below the level of the heart, in the surface ectoderm adjacent to the labelled gut mesoderm.
TS15: surface ectoderm	Present	Regionally restricted	5J/K	Expression is detected in the gut associated mesoderm at and below the level of the heart, in the surface ectoderm adjacent to the labelled gut mesoderm.
TS15: tail unsegmented mesenchyme	Present		5J/K
TS15: nephric duct	Present		5J/K
TS15: neural tube	Present	Graded	5J/K	Expression is detected in the neural tube in a posterior to anterior gradient.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present	Regionally restricted	6A/B left	Expression is detected in Rhombomere 4 but is absent in the floorplate.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present	Regionally restricted	6A/B center	Expression is detected in Rhombomere 4 but is absent in the floorplate.
TS17: rhombomere 4 floor plate	Absent		6A/B center

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present	Regionally restricted	6A/B right	Expression is detected in Rhombomere 4 but is absent in the floorplate.
TS17: rhombomere 4 floor plate	Absent		6A/B right

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Weak		7B

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Absent		7E
TS19: hindbrain	Weak		7E

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Graded		Expression is detected in the neural tube in a dorsoventral gradient.

Structure	Level	Pattern	Image	Note
TS20: embryo	Absent