Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:65597
van Raamsdonk CD, et al., Dosage requirement and allelic expression of PAX6 during lens placode formation. Development. 2000 Dec;127(24):5439-48
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:1928218
|
|
Gene symbol:
|
Sox2
|
|
Gene name:
|
SRY (sex determining region Y)-box 2
|
|
Antibody:
|
anti-SOX2
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
|
Results
|
Specimen
6C:
embryonic day 9.5
(more  )
Note: 24 somites.
Specimen
6C:
(close  )
|
Genetic Background:
|
C3H/HeJ
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Methanol
|
|
Embedding:
|
Paraffin
|
|
Note:
|
24 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: surface ectoderm |
Strong |
Regionally restricted |
6C
|
Expression is in the preplacode ectoderm.
|
Specimen
6D:
embryonic day 9.5; Pax6Sey-Neu/Pax6 +
(more )
Note: 24 somites.
Specimen
6D:
(close )
|
Genetic Background:
|
C3.Cg-Pax6Sey-Neu
|
|
Mutant Allele(s):
|
Pax6Sey-Neu/Pax6+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Methanol
|
|
Embedding:
|
Paraffin
|
|
Note:
|
24 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: surface ectoderm |
Strong |
Regionally restricted |
6D
|
Expression is in the preplacode ectoderm. Expression in this mutant is the same as in wild-type embryos.
|
|
Analysis Tools
