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Gene Expression Data
Assay Details
Assay
Reference: J:65599 Gaufo GO, et al., Hoxb1 controls effectors of sonic hedgehog and mash1 signaling pathways. Development. 2000 Dec;127(24):5343-54
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1928143
Gene symbol: Ascl1
Gene name: achaete-scute family bHLH transcription factor 1
Antibody: anti Mash1
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 3g: embryonic day 11.5 (more )
Note: Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 Present Regionally restricted 3g Expression was restricted to the inner ventricular neuroepithelium of the ventral progenitor domain.
Specimen 3h: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript.
Structure Level Pattern Image Note
TS18: rhombomere 4 Present Regionally restricted 3h Expressing progenitor cells have expanded into the mantle layer.
Specimen 7c,e,g,i,k: embryonic day 11.5 (more )
Note: Double labeled: green - Ascl1; red - Hoxb1 (assay MGI:1928087). Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript. (e,g,i,k) are higher magnifications of specific areas in (c).
Structure Level Pattern Image Note
TS18: rhombomere 4 Present Regionally restricted 7c , 7e , 7g , 7k , 7i Expression is in neuronal columns.
Specimen 7d,f,h,j,l: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Theiler stage 18 was chosen to remain consistent with other annotations from this manuscript. (f,h,j,l) are higher magnifications of specific areas in (d).
Structure Level Pattern Image Note
TS18: rhombomere 4 Present Regionally restricted 7f , 7l , 7j , 7h , 7d In this mutant, the expression domain has expanded into the domain formerly occupied by Hoxb1-expressing postmitotic neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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