Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63641
Vivian JL, et al., Thoracic skeletal defects in myogenin- and MRF4-deficient mice correlate with early defects in myotome and intercostal musculature. Dev Biol. 2000 Aug 1;224(1):29-41
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1913098
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Gene symbol:
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Des
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Gene name:
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desmin
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Antibody:
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ZC18
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
5A:
embryonic day 11.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail somite |
Present |
Regionally restricted |
5A
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Expression in myotome.
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Specimen
5B:
(close )
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Genetic Background:
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either: B6.129S7-Myf6tm1Eno or (involves: 129S7/SvEvBrd * C57BL/6)
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Mutant Allele(s):
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Myf6tm1Eno/Myf6tm1Eno
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail somite |
Weak |
Regionally restricted |
5B
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Limited expression in the medial portion of the myotome.
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Specimen
5C:
(close )
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Genetic Background:
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either: C57BL/6 or (involves: 129S7/SvEvBrd * C57BL/6)
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Mutant Allele(s):
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Myf6tm1Eno/Myf6tm1Eno Myogtm2Whk/Myogtm2Whk
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail somite |
Weak |
Regionally restricted |
5C
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Limited expression in the medial portion of the myotome.
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Specimen
6A:
embryonic day 11.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail somite |
Present |
Regionally restricted |
6A
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Expression in intercostal muscles.
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| TS19: trunk somite |
Present |
Regionally restricted |
6A
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Expression in intercostal muscles.
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Specimen
6B:
(close )
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Genetic Background:
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either: B6.129S7-Myf6tm1Eno or (involves: 129S7/SvEvBrd * C57BL/6)
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Mutant Allele(s):
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Myf6tm1Eno/Myf6tm1Eno
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail somite |
Weak |
Regionally restricted |
6B
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Reduced expression in internal region of intercostal muscles.
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| TS19: trunk somite |
Weak |
Regionally restricted |
6B
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Reduced expression in internal region of intercostal muscles.
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Specimen
6C:
(close )
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Genetic Background:
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either: C57BL/6 or (involves: 129S7/SvEvBrd * C57BL/6)
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Mutant Allele(s):
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Myf6tm1Eno/Myf6tm1Eno Myogtm2Whk/Myogtm2Whk
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail somite |
Weak |
Regionally restricted |
6C
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Reduced expression in internal region of intercostal muscles.
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| TS19: trunk somite |
Weak |
Regionally restricted |
6C
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Reduced expression in internal region of intercostal muscles.
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