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Gene Expression Data
Assay Details
Assay
Reference: J:64329 Welsh IC, et al., Loss of late primitive streak mesoderm and interruption of left-right morphogenesis in the Ednrb(s-1Acrg) mutant mouse. Dev Biol. 2000 Sep 1;225(1):151-68
Assay type: RNA in situ
MGI Accession ID: MGI:1891736
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 genomic4
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 8B: embryonic day 8.0; Ednrbs-1Acrg/Ednrbs-1Acrg (more )
Structure Level Pattern Image Note
TS11: amniotic cavity Present Single cells 8B Expression is detected in detached clusters of cells in the amniotic cavity.
Specimen not shown: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted Expression is in ectodermal cells lateral to the streak.
TS11: primitive streak Present Regionally restricted Expression is detected in epithelial cells of streak.
Specimen not shown: embryonic day 7.5; Ednrbs-1Acrg/Ednrbs-1Acrg (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted Comparable to wild type, expression is detected in ectodermal cells lateral to the streak.
TS11: primitive streak Present Regionally restricted Comparable to wild type, expression is detected in epithelial cells of streak.
Specimen 10A WT: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: head paraxial mesenchyme Present Regionally restricted 10A WT Expression is detected in the anterior paraxial mesoderm.
TS12: trunk mesenchyme Present Regionally restricted 10A WT Expression is detected in the nascent mesoderm emerging from the streak.
Specimen 10A -/-: embryonic day 8.0; Ednrbs-1Acrg/Ednrbs-1Acrg (more )
Structure Level Pattern Image Note
TS12: head paraxial mesenchyme Absent 10A -/-
TS12: trunk mesenchyme Absent 10A -/- Expression is absent in the nascent mesoderm emerging from the streak.
Specimen 10B, D: embryonic day 8.5 (more )
Note: Specimens B and D are side and posterior views of the same embryo.
Structure Level Pattern Image Note
TS13: midbrain-hindbrain junction Present 10D , 10B
TS13: trunk mesenchyme Present Regionally restricted 10B , 10D Expression is detected in the mesendoderm underlying the primitive streak, and mesoderm migrating away from the primitive streak.
TS13: primitive streak Present Regionally restricted 10B , 10D Expression was detected in streak epithelium.
TS13: embryo ectoderm Present Regionally restricted 10B , 10D Expression was detected in ectoderm lateral to the streak.
Specimen 10C, E: embryonic day 8.5; Ednrbs-1Acrg/Ednrbs-1Acrg (more )
Note: Specimens C and E are side and posterior views of the same embryo.
Structure Level Pattern Image Note
TS13: midbrain-hindbrain junction Trace 10C , 10E
TS13: trunk mesenchyme Absent 10E , 10C Expression is absent in the mesendoderm underlying the primitve streak and in the mesoderm migrating away from the primitive streak.
TS13: primitive streak Present Regionally restricted 10C , 10E Expression remained in the streak epithelium.
TS13: embryo ectoderm Present Regionally restricted 10E , 10C Expression remained in the ectoderm lateral to the streak.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory