Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64329
Welsh IC, et al., Loss of late primitive streak mesoderm and interruption of left-right morphogenesis in the Ednrb(s-1Acrg) mutant mouse. Dev Biol. 2000 Sep 1;225(1):151-68
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1891734
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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T cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
7C wt:
embryonic day 8.0
(more  )
Specimen
7C wt:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochord |
Present |
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7C WT
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Expression is detected along the entire length of the notochord.
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Specimen
7C -/-:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochord |
Present |
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7C -/-
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Expression is detected along the entire length of the notochord.
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Specimen
7D:
embryonic day 9.5
(more )
Specimen
7D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7E:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
Regionally restricted |
7E
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Anterior notochord is malformed.
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| TS15: embryo mesenchyme |
Absent |
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7E
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Expression is stated as being absent in the posterior ventrolateral mesoderm.
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Specimen
8A:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: amniotic cavity |
Present |
Single cells |
8A
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Expression is detected in detached clusters of cells in the amniotic cavity.
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Specimen
not shown:
embryonic day 8.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9A:
embryonic day 8.75
(more )
Specimen
9A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9B:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: embryo mesenchyme |
Weak |
Regionally restricted |
9B
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Expression is reduced in mesoderm lateral to the streak.
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Specimen
9C:
embryonic day 9.0
(more )
Specimen
9C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: embryo mesenchyme |
Present |
Regionally restricted |
9C
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Expression is detected in the midline and in streak derived mesoderm.
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Specimen
9D:
(close )
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Genetic Background:
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involves: 101/Rl * C3H/Rl
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Mutant Allele(s):
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Ednrbs-1Acrg/Ednrbs-1Acrg
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: embryo mesenchyme |
Present |
Regionally restricted |
9D
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Expression continued in the midline but was lost in the streak derived mesoderm.
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Analysis Tools