Probe:	Bmp4 cDNA8
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	2A	Expression is detected in the anterior and posterior margins.

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region	Present		2B
TS20: hindlimb digit	Absent		2B

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit mesenchyme	Present	Regionally restricted	2C	Expression is in the prospective joint-forming regions of the digits and in the tarsal cartilage.
TS22: hindlimb interdigital region mesenchyme	Present		2C

Structure	Level	Pattern	Image	Note
TS22: fibula cartilage condensation	Present	Regionally restricted	2D	Expression is detected in the chondrocytes but not the perichondrium (shown is tibia).
TS22: hindlimb digit mesenchyme	Present	Regionally restricted	2D	Expression is detected in the chondrocytes but not the perichondrium (shown is tibia).
TS22: tibia cartilage condensation	Present	Regionally restricted	2D	Expression is detected in the chondrocytes but not the perichondrium (shown is tibia).