Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60986
Park HL, et al., Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation. Development. 2000 Apr;127(8):1593-605
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1354544
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5G:
embryonic day 11.5-12.5
(more  )
Specimen
5G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Present |
Regionally restricted |
5G
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Expression forms two ventrolateral stripes flanking the midline.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Present |
Regionally restricted |
5H
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Expression in this mutant extends into the midline.
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli1tm1Alj/Gli1tm1Alj
Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Analysis Tools
