About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:60095 Barrow JR, et al., Roles of Hoxa1 and Hoxa2 in patterning the early hindbrain of the mouse. Development. 2000 Mar;127(5):933-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1353303
Gene symbol: Hoxb1
Gene name: homeobox B1
Antibody: anti-Hoxb-1
Detection system: Secondary antibody coupled to Texas Red
Assay notes: In this assay expression is red.
Results
Specimen 1A: embryonic day 8.0 (more )
Note: 0 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 1A Expression is in the posterior neural tube with a precise anterior limit at the rhombomere 3/4 boundary.
Specimen 1B: embryonic day 8.0 (more )
Note: 2 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 1B Expression is in the rhombomere 4 region. The expression level is higher than in the 0-somite embryo.
Specimen 1C: embryonic day 8.0 (more )
Note: 3 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 1C The expression level increases in rhombomere 4.
Specimen 1D: embryonic day 8.0; Hoxa1tm1Mrc/Hoxa1tm2Mrc, Hoxa2tm1Mrc/Hoxa2+ (more )
Note: 2 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 1D The anterior limit of expression in this mutant is shifted caudally to the posterior end of the rhombomere 4 bulge.
Specimen 2B,C: embryonic day 8.0; Hoxa1tm3.1Mrc/Hoxa1+ (more )
Note: 2-3 somite-stage embryo. Double labeled: red - Hoxb1; green - Hoxa1 reporter (assay MGI:3799262).
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 2B , 2C The anterior boundary of expression was at the rhombomere 3/4 boundary.
Specimen 2E,F: embryonic day 8.0; Hoxa1tm3.1Mrc/Hoxa1tm3.1Mrc (more )
Note: 2-3 somite-stage embryo. Double labeled: red - Hoxb1; green - Hoxa1 reporter (assay MGI:3799262).
Structure Level Pattern Image Note
TS12: future hindbrain Present Regionally restricted 2E , 2F In this mutant the anterior limit of expression is shifted almost one rhombomere caudally from the rhombomere 3/4 boundary.
Specimen 3A: embryonic day 8.5; Hoxa1tm2Mrc/Hoxa1+, Hoxa2tm1Mrc/Hoxa2+ (more )
Note: 5 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS13: rhombomere 4 Strong 3A
Specimen 3B: embryonic day 8.5; Hoxa2tm1Mrc/Hoxa2tm1Mrc (more )
Note: 4-5 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS13: rhombomere 4 Strong 3B
TS13: rhombomere 3 Present Regionally restricted 3B Expression is in the lateral-most cells.
Specimen 3C: embryonic day 8.5; Hoxa1tm1Mrc/Hoxa1tm1Mrc (more )
Note: 5 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS13: rhombomere 5 Present 3C
TS13: rhombomere 4 Present Regionally restricted 3C Expression is caudal.
Specimen 3D: embryonic day 8.5; Hoxa1tm2Mrc/Hoxa1tm2Mrc, Hoxa2tm1Mrc/Hoxa2tm1Mrc (more )
Note: 5 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS13: rhombomere 5 Present 3D
TS13: rhombomere 4 Present Regionally restricted 3D Expression is caudal.
Specimen 3E: embryonic day 8.75; Hoxa1tm1Mrc/Hoxa1tm1Mrc (more )
Note: 6-7 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS13: future hindbrain posterior to rhombomere 5 neural crest Present 3E
TS13: rhombomere 5 Present 3E
TS13: rhombomere 4 Present Regionally restricted 3E The expression domain is reduced in this mutant. The anterior limit of expression is in posterior rhombomere 4.
Specimen 3F: embryonic day 8.75; Hoxa1tm2Mrc/Hoxa1tm2Mrc, Hoxa2tm1Mrc/Hoxa2tm1Mrc (more )
Note: 6-7 somite-stage embryo. Double labeled: red - Hoxb1; green - Egr2 (assay MGI:1353295).
Structure Level Pattern Image Note
TS13: rhombomere 4 Present Regionally restricted 3F The expression domain is reduced in this mutant. The anterior limit of expression is in posterior rhombomere 4. Expression extends into anterior rhombomere 5.
TS13: rhombomere 5 Present Regionally restricted 3F The expression domain is reduced in this mutant. The anterior limit of expression is in posterior rhombomere 4. Expression extends into anterior rhombomere 5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory