Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60092
Pfeffer PL, et al., Pax2 and homeodomain proteins cooperatively regulate a 435 bp enhancer of the mouse Pax5 gene at the midbrain-hindbrain boundary. Development. 2000 Mar;127(5):1017-28
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1353076
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Gene symbol:
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Pax5
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Gene name:
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paired box 5
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Probe:
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Pax5 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
8C Pax2+/-:
embryonic day 8.0; Pax2tm1Mbu/Pax2 +
(more  )
Note: 7-8 Somites.
Specimen
8C Pax2+/-:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H/He
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Mutant Allele(s):
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Pax2tm1Mbu/Pax2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7-8 Somites.
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Specimen
8C -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H/He
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Mutant Allele(s):
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Pax2tm1Mbu/Pax2tm1Mbu
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7-8 Somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future spinal cord neural plate |
Present |
Regionally restricted |
8C -/-
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Expression was delayed in the mutant compared to heterozygous embryo. Expression was not detected in the lateral region of the plate.
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Specimen
8D Pax2+/-:
embryonic day 8.5; Pax2tm1Mbu/Pax2 +
(more )
Note: 12 somites.
Specimen
8D Pax2+/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H/He
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Mutant Allele(s):
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Pax2tm1Mbu/Pax2+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somites.
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Specimen
8D -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C3H/He
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Mutant Allele(s):
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Pax2tm1Mbu/Pax2tm1Mbu
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somites.
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Analysis Tools
