Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:58081
Lettice L, et al., The dominant hemimelia mutation uncouples epithelial-mesenchymal interactions and disrupts anterior mesenchyme formation in mouse hindlimbs. Development. 1999 Nov;126(21):4729-36
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1349127
|
|
Gene symbol:
|
Shh
|
|
Gene name:
|
sonic hedgehog
|
|
Probe:
|
Shh probe17
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
2J +/+:
embryonic day 11.5
(more  )
Specimen
2J +/+:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2K Dh/+:
embryonic day 11.5; Dh/Dh +
(more )
Specimen
2K Dh/+:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dh/Dh+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2L Dh/Dh:
embryonic day 11.5; Dh/ Dh
(more )
Specimen
2L Dh/Dh:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dh/Dh
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: footplate mesenchyme |
Present |
Regionally restricted |
2L
|
Expression is in the posterior margin. In this mutant expression appears to extend apically, but this may be due to a slight curvature of the limb bud.
|
Specimen
2D +/+:
embryonic day 10.5
(more )
Specimen
2D +/+:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2E Dh/+:
embryonic day 10.5; Dh/Dh +
(more )
Specimen
2E Dh/+:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dh/Dh+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2F -/-:
embryonic day 10.5; Dh/ Dh
(more )
Specimen
2F -/-:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dh/Dh
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2M:
embryonic day 12.5; Dh/Dh +
(more )
Specimen
2M:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Mutant Allele(s):
|
Dh/Dh+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
2M
|
In this mutant there is an overgrowth of anterior tissue with ectopic expression.
|
|
Analysis Tools
