Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:41540
Rossel M, et al., Mice mutant for both Hoxa1 and Hoxb1 show extensive remodeling of the hindbrain and defects in craniofacial development. Development. 1999 Nov;126(22):5027-40
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:1346703
|
|
Gene symbol:
|
Crabp1
|
|
Gene name:
|
cellular retinoic acid binding protein I
|
|
Antibody:
|
anti-CRABP I
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
There are two Crabp1 antibodies. Either antibody may have been used in this assay. See MGI:4460229 for details about the second antibody.
|
|
|
Results
|
Specimen
5I:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1+
Hoxb1tm3Mrc/Hoxb1+
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: migrating neural crest |
Present |
Regionally restricted |
5I
|
CRABPI is present in three major neural crest cell populations emanating from r2, r4 and r6 migrating into the 3 branchial arches.
|
Specimen
5J:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1tm1Mrc
Hoxb1tm3Mrc/Hoxb1+
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: migrating neural crest |
Present |
Regionally restricted |
5J
|
Only two populations of migratory neural crest are labeled, one at the r2/r3 level and the second caudal to the otocyst, migrating into the 1st and 3rd arch, respectively.
|
Specimen
5K:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: migrating neural crest |
Present |
Regionally restricted |
5K
|
Only two populations of migratory neural crest are labeled, migrating into the 1st and 3rd arch.
|
Specimen
5L:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: migrating neural crest |
Present |
Regionally restricted |
5L
|
Only two populations of migratory neural crest are labeled, migrating into the 1st and 3rd arch.
|
Specimen
8F +/-;+/-:
embryonic day 9.5; Hoxa1tm1Mrc/Hoxa1 +, Hoxb1tm3Mrc/Hoxb1 +
(more  )
Note: 20-25 somites. Double labeled: green - Crabp1; red - TUNEL.
Specimen
8F +/-;+/-:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1+
Hoxb1tm3Mrc/Hoxb1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
20-25 somites. Double labeled: green - Crabp1; red - TUNEL.
|
Specimen
8F -/-;-/-:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Hoxa1tm1Mrc/Hoxa1tm1Mrc
Hoxb1tm3Mrc/Hoxb1tm3Mrc
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
20-25 somites. Double labeled: green - Crabp1; red - TUNEL.
|
|
Analysis Tools