Probe:	Shh cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	6A	Expression is seen in the ventral forebrain from the ventral hypothalamus continuously up to the preoptic area.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	6B	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6F , 6D	Shh expression has split in two, leaving a gap devoid of expression.
TS19: optic recess	Absent		6D , 6F	This gap in expression is the only interruption of the Shh expression domain along the A-P axis and corresponds to the optic recess.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6E , 6G	In Pax2 mutants, the gap, which is normallly devoid of Shh expression is lost, so that the Shh-expressing regions at the preoptic area and the ventral hypothalamus join together and there is no longer an interruption in Shh expression along the A-P axis.
TS19: optic recess	Present	Regionally restricted	6E , 6G	In Pax2 mutants, the gap, which is normallly devoid of Shh expression is lost, so that the Shh-expressing regions at the preoptic area and the ventral hypothalamus join together and there is no longer an interruption in Shh expression along the A-P axis.

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Present		6I	Some examples of ectopic Shh-expressing cells in the ventroproximal region of the optic stalks can be identified. This occurs in the absence of any obvious A-P extension of the normal Shh expression domains, in the preoptic area or the hypothalamus.