Probe:	Mo-10 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent		1B

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	2B	Transcripts were located in the ectoderm and mesoderm layers of the posterior half of the embryo.
TS11: embryo mesoderm	Present	Regionally restricted	2B	Transcripts were located in the ectoderm and mesoderm layers of the posterior half of the embryo.
TS11: allantois	Present		2B

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	3B	Transcripts were detected over the complete posterior half of the ectoderm and mesoderm layers.
TS11: embryo mesoderm	Present	Regionally restricted	3B	Transcripts were detected over the complete posterior half of the ectoderm and mesoderm layers.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		4B
TS12: embryo ectoderm	Present	Regionally restricted	4B	Transcripts were seen in the posterior regions of ectoderm and mesoderm. Both within ectoderm and mesoderm tissues, the anterior limits of transcripts extended anterior to the position of first somite formation.
TS12: embryo mesenchyme	Present	Regionally restricted	4B	Transcripts were seen in the posterior regions of ectoderm and mesoderm. Both within ectoderm and mesoderm tissues, the anterior limits of transcripts extended anterior to the position of first somite formation.

Structure	Level	Pattern	Image	Note
TS13: somite 02	Present		5B
TS13: somite 03	Present		5B
TS13: somite 04	Present		5B
TS13: somite 05	Present		5B
TS13: somite 06	Present		5B
TS13: somite 07	Present		5B
TS13: somite 08	Present		5B
TS13: future hindbrain	Present		5B

Structure	Level	Pattern	Image	Note
TS20: thyroid gland	Strong		6D
TS20: pharynx	Weak	Regionally restricted	6D	Less-intense labelling was seen generally over tissue which formed the floor of the pharynx.
TS20: thyroglossal duct	Weak		6D
TS20: tongue	Absent		6D
TS20: C1 vertebral cartilage condensation	Present	Regionally restricted	6D	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown.
TS20: T3 vertebral cartilage condensation	Present	Regionally restricted	6D	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown.
TS20: T4 vertebral cartilage condensation	Present	Regionally restricted	6D	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown.
TS20: T5 vertebral cartilage condensation	Present	Regionally restricted	6D	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown.
TS20: T6 vertebral cartilage condensation	Present	Regionally restricted	6D	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown.
TS20: T7 vertebral cartilage condensation	Present	Regionally restricted	6D	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown.
TS20: vertebral pre-cartilage condensation	Present	Regionally restricted	6D	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present	Regionally restricted	6F	The anterior boundary of expression is within the preotic hindbrain.
TS20: spinal cord	Present	Graded	6F	There is a gradual anterior-to-posterior reduction in the intensity of labelling along the length of the spinal cord.
TS20: C1 vertebral cartilage condensation	Present	Regionally restricted	6F	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown
TS20: T3 vertebral cartilage condensation	Present	Regionally restricted	6F	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown
TS20: T4 vertebral cartilage condensation	Present	Regionally restricted	6F	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown
TS20: T5 vertebral cartilage condensation	Present	Regionally restricted	6F	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown
TS20: T6 vertebral cartilage condensation	Present	Regionally restricted	6F	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown
TS20: T7 vertebral cartilage condensation	Present	Regionally restricted	6F	Transcripts were clearly present in the most anterior prevertebrae, including prevertebra 1 (C1).All prevertebrae were labelled; prevertebrae 10-14 (T3-T7) are shown
TS20: lung	Present	Regionally restricted	6F	Transcripts were restricted to mesodermal components and were not detected in the endodermally-derived lining epithelium.

Structure	Level	Pattern	Image	Note
TS20: liver	Absent		6H
TS20: mesonephros	Present	Uniform	6H
TS20: metanephros	Present	Uniform	6H
TS20: stomach	Present	Regionally restricted	6H	Transcripts were restricted to mesodermal components and were not detected in the endodermally-derived lining epithelium.

Structure	Level	Pattern	Image	Note
TS20: heart	Absent