Probe:	pm31.1e
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent		1A

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		2A

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		3A

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		4A
TS12: embryo ectoderm	Present	Regionally restricted	4A	Transcripts were seen in the posterior regions of ectoderm and mesoderm. The anterior limits of expression were situated posterior to the position of the earliest-developing somites.
TS12: embryo mesenchyme	Present	Regionally restricted	4A	Transcripts were seen in the posterior regions of ectoderm and mesoderm. The anterior limits of expression were situated posterior to the position of the earliest-developing somites.

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present	Regionally restricted	5A	Transcripts were restricted to ectoderm and mesoderm posterior to the region of early somite formation.
TS13: neural ectoderm	Present	Regionally restricted	5A	Transcripts were restricted to ectoderm and mesoderm posterior to the region of early somite formation.

Structure	Level	Pattern	Image	Note
TS20: thyroid gland	Absent		6C
TS20: pharynx	Absent		6C	Expression was absent from the floor of the pharynx.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present	Regionally restricted	6E	The anterior boundary is at the level of the fifth prevertebra in the ventral third of the spinal cord, and at about the level of the third prevertebra in the center of the spinal cord.
TS20: spinal cord	Present	Graded	6E	There is a gradual anterior-to-posterior reduction in the intensity of labelling along the length of the spinal cord.
TS20: axial skeleton	Present	Regionally restricted	6E	Transcripts were seen in prevertebra 12 (T12) but not in any of the more anterior preverterae. Prevertebrae 13-16 (T6-T9) were labelled strongly, and labelling persisted at a lower intensity over all more posterior prevertebrae.
TS20: lung	Absent		6E

Structure	Level	Pattern	Image	Note
TS20: liver	Absent		6G
TS20: mesonephros	Present	Uniform	6G
TS20: metanephros	Present	Uniform	6G
TS20: stomach	Absent		6G

Structure	Level	Pattern	Image	Note
TS20: heart	Absent