Probe:	Hoxa3 probe2
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS20: thyroid gland	Present		4D
TS20: lung	Present		4D
TS20: trachea	Present		4D
TS20: pharynx	Present	Regionally restricted	4D	Transcripts were detected in most of the tissues that formed the floor of the pharynx.

Structure	Level	Pattern	Image	Note
TS20: thyroglossal duct	Present		5A/B
TS20: thyroid gland	Present		5A/B
TS20: tongue	Absent		5A/B
TS20: outflow tract aortic component	Present	Regionally restricted	5A/B	Transcripts were detected within the walls of the aortic trunk.
TS20: heart	Present	Regionally restricted	5A/B	Transcripts were detected within a small patch of tissue restricted to the base of the heart.

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted		Transcripts were detected both within the walls of the aortic trunk and within a small patch of tissue restricted to the base of the heart.
TS20: outflow tract aortic component	Present	Regionally restricted		Transcripts were detected in the walls of the aortic trunk.
TS20: pharynx	Present	Regionally restricted		Expression was detected in ventral pharynx.
TS20: lung	Present
TS20: mesonephros	Present
TS20: metanephros	Present
TS20: thyroid gland	Present
TS20: trachea	Present
TS20: stomach	Present	Regionally restricted		Transcripts were restricted to the mesodermal components.
TS20: stomach epithelium	Absent

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	6A	The anterior boundary of lay in the anterior part of the floor of the myelencephalon. The bounday is irregular in shape along the dorsoventral axis of the nervous tissue. These irregularities have been found to be consistent among different embryos.
TS20: medulla oblongata floor plate	Present	Regionally restricted	6A	The anterior boundary of lay in the anterior part of the floor of the myelencephalon. The bounday is irregular in shape along the dorsoventral axis of the nervous tissue. These irregularities have been found to be consistent among different embryos.