Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45709
Melloy PG, et al., No turning, a mouse mutation causing left-right and axial patterning defects. Dev Biol. 1998 Jan 1;193(1):77-89
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1343326
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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brachyury probe10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 8.5
(more  )
Note: >7-10 somites.
Specimen
6A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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>7-10 somites.
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Specimen
6B:
embryonic day 8.5; nt/ nt
(more )
Note: >7-10 somites.
Specimen
6B:
(close )
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Genetic Background:
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CD-1
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Mutant Allele(s):
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nt/nt
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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>7-10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
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6B
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Expression in this mutant is the same as in wild-type embryos, except that expression indicates bifurcation and fragmentation of the notochord from the level of the heart to about the third somite.
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Specimen
6D:
embryonic day 8.0; nt/ nt
(more )
Note: <7-10 somites.
Specimen
6D:
(close )
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Genetic Background:
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CD-1
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Mutant Allele(s):
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nt/nt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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<7-10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: notochord |
Present |
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6D
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A continuous line of Brachyury expression along the axial midline indicates an intact notochord at this stage of development. Expression in this mutant is the same as in wild-type embryos.
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Analysis Tools
